Recombinant rat TNF alpha protein (ab9756)

Reviews (1)Q&A (3)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
      P16599
    • Species
      Rat
    • Sequence
      MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVFQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL
    • Amino acids
      80 to 235
    • Additional sequence information
      Corresponds to the mature soluble, biologically active form.

Specifications

Our Abpromise guarantee covers the use of ab9756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form
    Lyophilised
  • Additional notes
    The ED50, determined by the cytolysis of murine L929 cells in the presence of actinomycin D, is < 0.05 ng/ml, corresponding to a specific activity of > 5 x 107 units/mg.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    For lot specific reconstitution information please contact our Scientific Support Team.

General Info

  • Alternative names
    • APC1
    • APC1 protein
    • Cachectin
    • DIF
    • Differentiation inducing factor
    • Macrophage cytotoxic factor
    • Tnf
    • TNF superfamily, member 2
    • TNF, macrophage derived
    • TNF, monocyte derived
    • TNF-a
    • TNF-alpha
    • TNFA
    • TNFA_HUMAN
    • TNFSF2
    • Tumor necrosis factor
    • Tumor necrosis factor alpha
    • Tumor necrosis factor ligand superfamily member 2
    • Tumor Necrosis Factor, Membrane Form
    • Tumor necrosis factor, soluble form
    see all
  • Function
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in disease
    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt

References

ab9756 has not yet been referenced specifically in any publications.

Publishing research using ab9756? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

TNF-a effects on rat heart

 Excellent
Abreviews
abreview image
Application
Functional Studies
Rat heart was perfused 30min with Tyrode and TNF-a at 20ng/ml, using Langendorff system.

Mr. Baptiste Jude

Verified customer

Submitted Oct 07 2015

Question

could you please explain to me how much of water should add to the tube containing 20 micro gram of TNF alpha protein (code # ab9756 I just bought it) in order to make a 0.1 mg/ml of this product.

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Answer

For lot 851145: Centrifuge the vial prior to opening. Reconstitute in 200ul sterile water to a concentration of 0.1 mg/ml (or add 20ul for 1mg/ml). Do not vortex. This solution can be stored at 2-8oC for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20oC to -80oC.

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Question

I have been using ab9756 as a standard for western blotting. I keep getting 2 bands, one at 17kD and a second at 25kD. Is this 25kD band TNF-beta?

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Answer

I am terribly sorry about the delay replying to you regarding ab9756, I have had trouble contacting the source of this protein. I have just received confirmation that the protein gave only 1 band when tested in WB with the antibody ab9755. It is not likely that the second band is TNF beta but rather a non specific binding if you have used another antibody other than ab9755. Please accept my apologies for the delay in responding to you and do not hesitate to contact us if you need further information,

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Question

Ab9970 - Lyophilised from 5mM Tris, pH 7.2; how should it be reconstituted and with how much? Ab9756 - Lyophilized from 1x PBS; how should it be reconstituted and with how much?

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Answer

Thank you very much for your patience, I have just heard back from the originator of these proteins. Ab9756: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use. Ab9970: Centrifuge the vial prior to opening. Reconstitute in 5mM Tris, pH 7.2 to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use. Please let me know if you require any further information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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