Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant rat TXK/RLK protein
See all TXK/RLK proteins and peptides
The specific activity of ab207987 was determined to be 16 nmol/min/mg
Purity> 75 % Densitometry.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceYHKNITRDQTERLLRQEAKEGAFIVRDSRHLGSYTISVFTRARRHTQSSI KHYQIKKNDSGQWYVTERHLFPSVPELIQYHQYNAAGLMSRLRYPVGLLG SCLPATSGFSYEKWEIDPSELTFVKEIGSGQFGVVYLGEWRARIRVAIKA INEGSMSEEDFIEEAKVMMKLSHSRLVQLYGVCIQQKPLYIVTEFMENGC LLDYLRERKGKLPKALLLSMCQDICEGMAYLEKSCYIHRDLAARNCLVSS ACVVKISDFGMARYVLDDEYISSSGAKFPVKWSPPEVFHFNKYSSKSDVW SFGVLMWEVFTEGKMPFENKSNLQVVEAISKGFRLYRPHLAPMSIYGVMY SCWHESPKGRPTFAELLQVLAEIAETW
Predicted molecular weight64 kDa including tags
Amino acids190 to 526
TagsGST tag N-Terminus
Additional sequence informationBC095847
Our Abpromise guarantee covers the use of ab207987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product was previously labelled as TXK
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- EC 126.96.36.199
FunctionNon-receptor tyrosine kinase that plays a redundant role with ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of TXK to the cell membrane, where it is phosphorylated at Tyr-420. Phosphorylation leads to TXK full activation. Contributes also to signaling from many receptors and participates in multiple downstream pathways, including regulation of the actin cytoskeleton. Like ITK, can phosphorylate PLCG1, leading to its localization in lipid rafts and activation, followed by subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. With PARP1 and EEF1A1, TXK forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFNG to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Phosphorylates both PARP1 and EEF1A1. Phosphorylates also key sites in LCP2 leading to the up-regulation of Th1 preferred cytokine IL-2. Phosphorylates 'Tyr-201' of CTLA4 which leads to the association of PI-3 kinase with the CTLA4 receptor.
Tissue specificityExpressed in T-cells and some myeloid cell lines. Expressed in Th1/Th0 cells with IFN-gamma-producing potential.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
modificationsPhosphorylated at Tyr-420 by FYN. Autophosphorylation at Tyr-91 is critical for the activation of TXK, leading to the up-regulation of IFN-gamma gene transcription.
The cysteine string at the N-terminus is palmitoylated and required for the proper subcellular location.
Cellular localizationCytoplasm. Nucleus. Cell membrane. Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation. Translocates into the nucleus and enhances IFN-gamma gene transcription in T-cells.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab207987 has not yet been referenced specifically in any publications.