Description

  • Product name

    Recombinant REBOV GP protein
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Predicted molecular weight

      72 kDa
    • Additional sequence information

      Mature recombinant Reston ebolavirus glycoprotein (GP) minus the transmembrane domain.

Specifications

Our Abpromise guarantee covers the use of ab190132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    The theoretical molecular weight of the protein is ~72 kDa including the His tag, without glycosylation. Because of the glycosylated nature of this protein, migration in an SDS-PAGE gel results in a broad band.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C.

    Constituents: 89% PBS, 10% Glycerol

    Supplemented with arginine and glutamic acid.

General Info

  • Alternative names

    • pre-sGP
    • Pre-small/secreted glycoprotein
    • small/secreted non-structural glycoprotein
    • structural glycoprotein
  • Relevance

    REBOV GP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha. Might therefore contribute to the lack of inflammatory reaction seen during infection in spite the of extensive necrosis and massive virus production. Does not seem to be involved in activation of primary macrophages. Does not seem to interact specifically with neutrophils. Delta-peptide does not seem to be involved in activation of primary macrophages B REBOV GP is a homodimer; disulfide-linked. The homodimers are linked by two disulfide bonds in a parallel orientation. Delta-peptide is a monome
  • Cellular localization

    Secreted

Images

  • SDS-PAGE analysis of 1 µg (lanes 1 and 3) and 5 µg (lanes 2 and 4) of ab190132 under non-reducing (lanes 1 and 2) and reducing conditions (lanes 3 and 4).

  • All lanes : Rabbit anti-REBOV GP pAb at 0.5 µg/ml

    Lanes 1 & 4 : Recombinant REBOV GP protein (ab190132) at 0.5 µg
    Lanes 2 & 5 : Recombinant REBOV GP protein (ab190132) at 0.1 µg
    Lanes 3 & 6 : Recombinant REBOV GP protein (ab190132) at 0.05 µg

    Secondary
    All lanes : Anti-rabbit IgG-AP conjugate


    Nonreducing conditions (lanes 1-3) and reducing conditions (lanes 4-6).

References

ab190132 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab190132.
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