Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Tags: His tag C-Terminus
- Suitable for: WB, SDS-PAGE
Product nameRecombinant REBOV GP protein
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
Predicted molecular weight72 kDa
TagsHis tag C-Terminus
Additional sequence informationMature recombinant Reston ebolavirus glycoprotein (GP) minus the transmembrane domain.
Our Abpromise guarantee covers the use of ab190132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The theoretical molecular weight of the protein is ~72 kDa including the His tag, without glycosylation. Because of the glycosylated nature of this protein, migration in an SDS-PAGE gel results in a broad band.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C.
Constituents: 89% PBS, 10% Glycerol
Supplemented with arginine and glutamic acid.
- Pre-small/secreted glycoprotein
- small/secreted non-structural glycoprotein
- structural glycoprotein
RelevanceREBOV GP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha. Might therefore contribute to the lack of inflammatory reaction seen during infection in spite the of extensive necrosis and massive virus production. Does not seem to be involved in activation of primary macrophages. Does not seem to interact specifically with neutrophils. Delta-peptide does not seem to be involved in activation of primary macrophages B REBOV GP is a homodimer; disulfide-linked. The homodimers are linked by two disulfide bonds in a parallel orientation. Delta-peptide is a monome
SDS-PAGE analysis of 1 µg (lanes 1 and 3) and 5 µg (lanes 2 and 4) of ab190132 under non-reducing (lanes 1 and 2) and reducing conditions (lanes 3 and 4).
All lanes : Rabbit anti-REBOV GP pAb at 0.5 µg/ml
Lanes 1 & 4 :
Recombinant REBOV GP protein (ab190132) at 0.5 µg
Lanes 2 & 5 :
Recombinant REBOV GP protein (ab190132) at 0.1 µg
Lanes 3 & 6 :
Recombinant REBOV GP protein (ab190132) at 0.05 µg
All lanes : Anti-rabbit IgG-AP conjugate
Nonreducing conditions (lanes 1-3) and reducing conditions (lanes 4-6).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab190132 has not yet been referenced specifically in any publications.