Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, WB, Flow Cyt
Product nameRecombinant RFP protein
See all RFP proteins and peptides
Purity> 95 % SDS-PAGE.
The recombinant RFP (Red Fluorescence Protein) is expressed and purified from transformed E. coli using a method that ensures high purity and maximal fluorescence of the protein.
Endotoxin was removed for in vivo injection or cell culture applications. Endotoxin level equal to or less than 0.1 ng/µg.
Expression systemEscherichia coli
Protein lengthFull length protein
Predicted molecular weight28 kDa including tags
TagsHis tag N-Terminus
Our Abpromise guarantee covers the use of ab51993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product is manufactured by BioVision, an Abcam company and was previously called 4997 dsRed Fluorescent Protein. 4997-100 is the same size as the 100 µg size of ab51993.
This protein (DSRed) is suitable as control reagent for RFP expression studies or as a labeling reagent.
Endotoxin level is <0.1 ng/μg.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
ReconstitutionReconstitute with 100 µl dH2O to 1mg/ml
- GFP like chromoprotein
- red fluorescent protein
RelevanceFluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab51993 has been referenced in 1 publication.
- Dufour YS et al. Direct Correlation between Motile Behavior and Protein Abundance in Single Cells. PLoS Comput Biol 12:e1005041 (2016). PubMed: 27599206