Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: ELISA, SDS-PAGE
Product nameRecombinant rhesus monkey CD4 protein (His tag)
See all CD4 proteins and peptides
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rhesus monkey CD4 protein (His Tag) (ab208305) at 1 μg/mL (100 μL/well) can bind Ibalizumab with a linear range of 0.2-13 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized Ibalizumab at 1 μg/mL (100 μL/well) can bind Recombinant Rhesus monkey CD4 protein (His Tag) (ab208305) with a linear range of 0.8-13 ng/mL.
Purity> 95 % SDS-PAGE.
Endotoxin level< 1.000 Eu/µg
Expression systemHEK 293 cells
Protein lengthProtein fragment
SequenceKKVVL GKKGDTVELT CNASQKKNTQ FHWKNSNQIK ILGIQGSFLT KGPSKLSDRA DSRKSLWDQG CFSMIIKNLK IEDSDTYICE VENKKEEVEL LVFGLTANSD THLLEGQSLT LTLESPPGSS PSVKCRSPGG KNIQGGRTIS VPQLERQDSG TWTCTVSQDQ KTVEFKIDIV VLAFQKASST VYKKEGEQVE FSFPLAFTLE KLTGSGELWW QAERASSSKS WITFDLKNKE VSVKRVTQDP KLQMGKKLPL HLTLPQALPQ YAGSGNLTLA LEAKTGKLHQ EVNLVVMRAT QFQENLTCEV WGPTSPKLTL SLKLENKGAT VSKQAKAVWV LNPEAGMWQC LLSDSGQVLL ESNIKVVPTW
Predicted molecular weight42 kDa including tags
Amino acids26 to 390
TagsHis tag C-Terminus
Our Abpromise guarantee covers the use of ab208305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
Constituent: 97% PBS
Note: 5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
- CD 4
- CD4 (L3T4)
FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
- Information by UniProt
SDS-PAGE analysis of reduced ab208305 (Lane 1) stained overnight with Coomassie Blue. The protein migrates as 50-57 kDa under reducing condition due to glycosylation.
Immobilized Recombinant Rhesus monkey CD4 protein (His Tag) (ab208305) at 1 μg/mL (100 μL/well) can bind Ibalizumab with a linear range of 0.2-13 ng/mL.
Immobilized Ibalizumab at 1 μg/mL (100 μL/well) can bind Recombinant Rhesus monkey CD4 protein (His Tag) (ab208305) with a linear range of 0.8-13 ng/mL.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab208305 has been referenced in 1 publication.
- Chen Q et al. Shenyan Kangfu tablet alleviates diabetic kidney disease through attenuating inflammation and modulating the gut microbiota. J Nat Med 75:84-98 (2021). PubMed: 32997272