Recombinant rhesus monkey Folate Binding Protein/FBP (Active) (ab286067)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant rhesus monkey Folate Binding Protein/FBP (Active)
See all Folate Binding Protein/FBP proteins and peptides -
Biological activity
Immobilized Folic acid-BSA conjugate at 20 µg/mL (100 µL/well) can bind Rhesus macaque FOLR1, with a linear range of 0.156-2.5 µg/mL
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Purity
>= 90 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Rhesus monkey -
Predicted molecular weight
26 kDa including tags -
Actual molecular weight
35 kDa including tags -
Molecular weight information
The protein has a calculated MW of 26.4 kDa. The protein migrates as 35-41 kDa under reducing conditions (SDS-PAGE) due to glycosylation. -
Amino acids
25 to 233 -
Tags
His tag C-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab286067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: PBS
Normally Mannitol or Trehalose is added as protectants before lyophilization.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute in sterile deionized water to a concentration of 50 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% (W/V) HSA or BSA) is recommended for further dilution and long term storage. Do not vortex. After reconstitution, aliquot and store at -80°C and use within 3 months.
General Info
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Alternative names
- adult
- Adult folate binding protein
- Adult folate-binding protein
see all -
Function
Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. -
Tissue specificity
Exclusively expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum. -
Involvement in disease
Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD) [MIM:613068]. NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Note=Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function. -
Sequence similarities
Belongs to the folate receptor family. -
Post-translational
modificationsEight disulfide bonds are present.
The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease. -
Cellular localization
Cell membrane. Secreted. - Information by UniProt
Images
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Functional Studies - Recombinant rhesus monkey Folate Binding Protein/FBP (Active) (ab286067)
Immobilized Folic acid-BSA conjugate at 20 μg/mL (100 μL/well) can bind Rhesus macaque FOLR1, with a linear range of 0.156-2.5 μg/mL
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SDS-PAGE - Recombinant rhesus monkey Folate Binding Protein/FBP (Active) (ab286067)SDS-PAGE analysis of ab286067 under reducing conditions (R).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab286067 has not yet been referenced specifically in any publications.
