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Bioactive grade

Recombinant rhesus monkey GM-CSF protein (Active) (ab243115)

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SDS-PAGE - Recombinant rhesus monkey GM-CSF protein (Active) (ab243115)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 98% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Active: Yes
    • Suitable for: Functional Studies, SDS-PAGE, HPLC

    Description

    • Product name

      Recombinant rhesus monkey GM-CSF protein (Active)
      See all GM-CSF proteins and peptides
    • Biological activity

      Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of >1.0x107 IU/mg.

    • Purity

      > 98 % SDS-PAGE.
      > 98 % by HPLC.
    • Endotoxin level

      < 1.000 Eu/µg
    • Expression system

      Escherichia coli
    • Accession

      Q9GL44
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Rhesus monkey
      • Sequence

        APARSPSPGTQPWEHVNAIQEARRLLNLSRDTAAEMNKTVEVVSEMFDLQ EPSCLQTRLELYKQGLQGSLTKLKGPLTMMASHYKQHCPPTPETSCATQI ITFQSFKENLKDFLLVIPFDCWEPVQE
      • Predicted molecular weight

        16 kDa
      • Amino acids

        18 to 144
      • Additional sequence information

        This product is the mature full length protein from aa 18 to 144. The signal peptide is not included.

    Specifications

    Our Abpromise guarantee covers the use of ab243115 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      SDS-PAGE

      HPLC

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Constituent: PBS

      0.2 µm filtered.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Briefly centrifuge prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20 °C or below. Further dilutions should be made in appropriate buffered solutions.

    General Info

    • Alternative names

      • Colony stimulating factor
      • Colony Stimulating Factor 2
      • Colony stimulating factor 2 (granulocyte-macrophage)
      • Colony-stimulating factor
      • CSF
      • CSF 2
      • CSF2
      • CSF2_HUMAN
      • GM-CSF
      • GMCSF
      • Granulocyte Macrophage Colony Stimulating Factor
      • Granulocyte-macrophage colony-stimulating factor
      • MGC131935
      • MGC138897
      • MGI1GM
      • Molgramostin
      • Pluripoietin-a
      • Sargramostim
      see all
    • Function

      Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
    • Sequence similarities

      Belongs to the GM-CSF family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P04141 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant rhesus monkey GM-CSF protein (Active) (ab243115)
      SDS-PAGE - Recombinant rhesus monkey GM-CSF protein (Active) (ab243115)
      SDS Page analysis of ab243115

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab243115? Please let us know so that we can cite the reference in this datasheet.

    ab243115 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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