Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant Rubella Virus spike glycoprotein E1 protein (Fc Chimera)
Purity>= 95 % SDS-PAGE.
Buffered in DPBS, pH7.4.
Expression systemHEK 293 cells
Protein lengthProtein fragment
SequenceEEAFTYLCTAPGCATQAPVPVRLAGVRFESKIVDGGCFAPWDLEATGACI CEIPTDVSCEGLGAWVPAAPCARIWNGTQRACTFWAVNAYSSGGYAQLAS YFNPGGSYYKQYHPTACEVEPAFGHSDAACWGFPTDTVMSVFALASYVQH PHKTVRVKFHTETRTVWQLSVAGVSCNVTTEHPFCNTPHGQLEVQVPPDP GDLVEYIMNYTGNQQSRWGLGSPNCHGPDWASPVCQRHSPDCSRLVGATP ERPRLRLVDADDPLLRTAPGPGEVWVTPVIGSQARKCGLHIRAGPYGHAT VEMPEWIHAHTTSDPWHPPGPLGLKFKTVRPVALPRTLAPPRNVRVTGCY QCGTPALVEGLAPGGGNCHLTVNGEDLGAVPPGKFVTAALLNTPPPYQVS CGGESDRATARVIDPAAQSFTGVVYGTHTTAVSETRQTWAEWA
Amino acids583 to 1025
TagsFc tag C-Terminus
Additional sequence information16 amino acid Gly-Ser linker connecting protein to sheep IgG Fc (CH2-CH3). NP_062884.1
DescriptionRecombinant Rubella virus E1 protein (Fc Chimera)
Our Abpromise guarantee covers the use of ab256429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: PBS, 0.1% CHAPS
0.2 micron filtered
- E1 envelope glycoprotein
- Glycoprotein E1
- Spike glycoprotein E1
RelevanceRubella virus is the only member of the Rubrivirus genus of the Togavirus family. Unlike most Togaviruses it is NOT arthropod borne, but is acquired via the respiratory route. It is an enveloped (toga=cloak), non-segmented, positive sense, RNA virus and replicates in the cytoplasm. It consists of 3 structural proteins; E1,E2 membrane bound glycoproteins, and C capsid protein. E1 envelope glycoprotein is a class II viral fusion protein. Fusion activity is inactive as long as E1 is bound to E2 in mature virion. After virus attachment to target cell and endocytosis, acidification of the endosome induces dissociation of E1/E2 heterodimer and trimerization of the E1 subunits. This E1 homotrimer is fusion active, and promotes release of viral nucleocapsid in the cytoplasm after cell and viral membrane fusion. The E1 cytoplasmic tail modulates virus release, and the tyrosines residues are critical for this function.
SDS-PAGE analysis of 3.2 μg (Lane 1) and 1.7 μg (Lane 2) of ab256429 under reducing conditions.
Detection of anti-Rubella IgG in human serum.
Plate coated with 50 ng/well of antigens. E1 = ab256429
Antigens coated in bicarbonate-carbonate buffer pH 9.6 for 1 hour at RT. Blocked with 2% BSA/PBS for 2 hours at RT.
Washed x3 with Tris washing buffer.
Serum samples (Public Health England) diluted 1/201 in 1% BSA in PBS-T.
Secondary antibody was anti-Human-IgG-HRP diluted 1/10000 in 1% BSA in PBS-T. TMB detection.
Detection of anti-Rubella IgM in human serum.
Plate coated with 100 ng/well of antigens. E1 = ab256429
Washed x3 with Tris washing buffer.
Serum samples (Public Health England) diluted 1/201 in 1% BSA in PBS-T + 4% IgG/RF stripper. After standing for 30 minutes the diluted samples were centrifuged at 17,000 x g for 1 minute and the supernatant used for ELISA.
Secondary antibody was anti-Human-IgM-HRP diluted 1/10000 in 1% BSA in PBS-T. TMB detection.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab256429 has not yet been referenced specifically in any publications.