Description

  • Product name

    Recombinant S. cerevisiae GAL4 protein
    See all GAL4 proteins and peptides
  • Purity

    > 95 % SDS-PAGE.
    ab81879 is isolated from an E. coli strain that carries the coding sequence of the fused protein under the control of a T7 promoter.
  • Expression system

    Escherichia coli
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Saccharomyces cerevisiae
    • Amino acids

      1 to 147

Specifications

Our Abpromise guarantee covers the use of ab81879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Additional notes


    Transcriptional activity is greatly stimulated by promoter-specific activator proteins. These are modular proteins, consisting of a DNA-binding domain and a regulatory (activator) domain. The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5`-CGGN5(T/A)N5CCG-3`. A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. Linking of an acidic synthetic peptide, forming an {alpha}-helix, to this GAL4 DNA-binding domain, results in a protein with an amphipathic structure. This fusion protein is able to activate transcription of a gene, bearing the GAL4 binding sites in an in vitro transcription system by targeting TFIIB in the pre-initiation complex.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol

General Info

  • Alternative names

    • Antigen NY CO 27
    • Antigen NY-CO-27
    • Antigen NYCO27
    • GAL 4
    • Gal-4
    • GAL4
    • Galectin 4
    • Galectin-4
    • Galectin4
    • Homo sapiens galectin4 mRNA complete cds
    • L 36 lactose binding protein
    • L-36 lactose-binding protein
    • L36 lactose binding protein
    • L36LBP
    • Lactose binding lectin 4
    • Lactose-binding lectin 4
    • Lectin galactoside binding soluble 4
    • LEG4_HUMAN
    • LGALS4
    see all
  • Function

    Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.
  • Sequence similarities

    Contains 2 galectin domains.
  • Domain

    Contains two homologous but distinct carbohydrate-binding domains.
  • Information by UniProt

References

ab81879 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

The GAL4 –AH (P1018) has a fragment 1-147 aa and an additiona alpha helix sequence (denoted AH).

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Question
Answer

Thank you for contacting us.

In the absence of the alpha helix, the GAL4 protein willbind to DNA but will not activate transcription. The alpha helix allows the protein complex to activate genes involved in galactose metabolism.


I hope this helps, please let me know if you need any additional information or assistance.

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