Description

  • Product name

    Recombinant S. cerevisiae Glutaredoxin 1 protein
  • Biological activity

     

    Strongly binds to Glutathione, reduced and oxidized.

  • Purity

    > 90 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Saccharomyces cerevisiae
    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab63231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Preservative: 0.01% Sodium azide
    Constituent: 0.395% Tris HCl

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

References

This product has been referenced in:

  • Yao F  et al. Low frequency pulsed electromagnetic field promotes differentiation of oligodendrocyte precursor cells through upregulation of miR-219-5p in vitro. Life Sci 223:185-193 (2019). Read more (PubMed: 30885522) »
  • Park HA  et al. Glutathione disulfide induces neural cell death via a 12-lipoxygenase pathway. Cell Death Differ 16:1167-79 (2009). Read more (PubMed: 19373248) »
See all 2 Publications for this product

Customer reviews and Q&As

Question
Answer

The Glutaredoxin 1 protein (Active), ab63231, is the Glutaredoxin 1 protein from Saccharomyces cerevisiae (SwissProt reference P25373).
The activity of the protein was ascertained by monitoring its ability to oxidize NADPH. The activity was calculated to be 3X10^-3 micromol/min. The reaction mix contained NADPH (0.4 mM), GSH (1.0 mM), glutathione reductase (17 ug) and HED (1.4 mM) in 0.1 M Tris HCl, pH 7.4.
A mixed disulphide between HED and GSH is formed within 2 minutes, and the reaction was started by the addition of 17 ug Grx1. The reaction was followed by the decrease in A340 due to the oxidation of NADPH.

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