Recombinant S. cerevisiae HSP82 protein (ab157058)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 99% n/a
- Tags: His tag N-Terminus
- Suitable for: WB
Description
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Product name
Recombinant S. cerevisiae HSP82 protein -
Purity
>= 99 % n/a. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Saccharomyces cerevisiae -
Sequence
MASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSL SDPKQLETEPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSG TKAFMEALSAGADVSMIGQFGVGFYSLFLVADRVQVISKSNDDEQYIWES NAGGSFTVTLDEVNERIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEF VAYPIQLVVTKEVEKEVPIPEEEKKDEEKKDEEKKDEDDKKPKLEEVDEE EEKKPKTKKVKEEVQEIEELNKTKPLWTRNPSDITQEEYNAFYKSISNDW EDPLYVKHFSVEGQLEFRAILFIPKRAPFDLFESKKKKNNIKLYVRRVFI TDEAEDLIPEWLSFVKGVVDSEDLPLNLSREMLQQNKIMKVIRKNIVKKL IEAFNEIAEDSEQFEKFYSAFSKNIKLGVHEDTQNRAALAKLLRYNSTKS VDELTSLTDYVTRMPEHQKNIYYITGESLKAVEKSPFLDALKAKNFEVLF LTDPIDEYAFTQLKEFEGKTLVDITKDFELEETDEEKAEREKEIKEYEPL TKALKEILGDQVEKVVVSYKLLDAPAAIRTGQFGWSANMERIMKAQALRD SSMSSYMSSKKTFEISPKSPIIKELKKRVDEGGAQDKTVKDLTKLLYETA LLTSGFSLDEPTSFASRINRLISLGLNIDEDEETETAPEASTAAPVEEVP ADTEMEEVD -
Predicted molecular weight
81 kDa -
Amino acids
1 to 709 -
Tags
His tag N-Terminus
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Description
Recombinant S. cerevisiae HSP82 protein
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab157058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
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Form
Liquid -
Additional notes
For maximum product recovery after thawing, centrifuge the vial before opening the cap. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituent: 1.12% Potassium chloride
General Info
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Alternative names
- 82 kDa heat shock protein
- ATP-dependent molecular chaperone HSP82
- Heat shock protein Hsp90 heat-inducible isoform
see all -
Relevance
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. The nucleotide-free form of the dimer is found in an open conformation in which the N-termini are not dimerized and the complex is ready for client protein binding. Binding of ATP induces large conformational changes, resulting in the formation of a ring-like closed structure in which the N-terminal domains associate intramolecularly with the middle domain and also dimerize with each other, stimulating their intrinsic ATPase activity and acting as a clamp on the substrate. Finally, ATP hydrolysis results in the release of the substrate. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Required for growth at high temperatures. -
Cellular localization
Cytoplasmic
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab157058 has not yet been referenced specifically in any publications.