Recombinant S. cerevisiae Hsp90 protein (ab157271)
Key features and details
- Expression system: Escherichia coli
- Tags: His tag N-Terminus
- Suitable for: WB
Description
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Product name
Recombinant S. cerevisiae Hsp90 protein -
Purity
>= 99 % . -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Saccharomyces cerevisiae -
Sequence
MASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSL SDPKQLETEPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSG TKAFMEALSAGADVSMIGQFGVGFYSLFLVADRVQVISKSNDDEQYIWES NAGGSFTVTLDEVNERIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEF VAYPIQLVVTKEVEKEVPIPEEEKKDEEKKDEEKKDEDDKKPKLEEVDEE EEKKPKTKKVKEEVQEIEELNKTKPLWTRNPSDITQEEYNAFYKSISNDW EDPLYVKHFSVEGQLEFRAILFIPKRAPFDLFESKKKKNNIKLYVRRVFI TDEAEDLIPEWLSFVKGVVDSEDLPLNLSREMLQQNKIMKVIRKNIVKKL IEAFNEIAEDSEQFEKFYSAFSKNIKLGVHEDTQNRAALAKLLRYNSTKS VDELTSLTDYVTRMPEHQKNIYYITGESLKAVEKSPFLDALKAKNFEVLF LTDPIDEYAFTQLKEFEGKTLVDITKDFELEETDEEKAEREKEIKEYEPL TKALKEILGDQVEKVVVSYKLLDAPAAIRTGQFGWSANMERIMKAQALRD SSMSSYMSSKKTFEISPKSPIIKELKKRVDEGGAQDKTVKDLTKLLYETA LLTSGFSLDEPTSFASRINRLISLGLNIDEDEETETAPEASTAAPVEEVP ADTEMEEVD -
Predicted molecular weight
82 kDa -
Amino acids
1 to 709 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab157271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
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Form
Liquid -
Additional notes
For maximum product recovery after thawing, centrifuge the vial before opening the cap. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 1.12% Potassium chloride, 0.95% HEPES
General Info
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Alternative names
- Heat shock 86 kDa
- Heat shock protein 90kDa alpha cytosolic class A member 1
- Heat shock protein 90kDa alpha cytosolic class B member 1
see all -
Function
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. -
Sequence similarities
Belongs to the heat shock protein 90 family. -
Domain
The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1. -
Post-translational
modificationsISGylated.
S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab157271 has not yet been referenced specifically in any publications.