Recombinant S. cerevisiae Hsp90 protein (ab157271)

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Key features and details

  • Expression system: Escherichia coli
  • Tags: His tag N-Terminus
  • Suitable for: WB

Description

  • Product name

    Recombinant S. cerevisiae Hsp90 protein

  • Purity

    >= 99 % .

  • Expression system

    Escherichia coli

  • Accession

    P02829

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Saccharomyces cerevisiae

    • Sequence

      MASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSL SDPKQLETEPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSG TKAFMEALSAGADVSMIGQFGVGFYSLFLVADRVQVISKSNDDEQYIWES NAGGSFTVTLDEVNERIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEF VAYPIQLVVTKEVEKEVPIPEEEKKDEEKKDEEKKDEDDKKPKLEEVDEE EEKKPKTKKVKEEVQEIEELNKTKPLWTRNPSDITQEEYNAFYKSISNDW EDPLYVKHFSVEGQLEFRAILFIPKRAPFDLFESKKKKNNIKLYVRRVFI TDEAEDLIPEWLSFVKGVVDSEDLPLNLSREMLQQNKIMKVIRKNIVKKL IEAFNEIAEDSEQFEKFYSAFSKNIKLGVHEDTQNRAALAKLLRYNSTKS VDELTSLTDYVTRMPEHQKNIYYITGESLKAVEKSPFLDALKAKNFEVLF LTDPIDEYAFTQLKEFEGKTLVDITKDFELEETDEEKAEREKEIKEYEPL TKALKEILGDQVEKVVVSYKLLDAPAAIRTGQFGWSANMERIMKAQALRD SSMSSYMSSKKTFEISPKSPIIKELKKRVDEGGAQDKTVKDLTKLLYETA LLTSGFSLDEPTSFASRINRLISLGLNIDEDEETETAPEASTAAPVEEVP ADTEMEEVD

    • Predicted molecular weight

      82 kDa

    • Amino acids

      1 to 709

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab157271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

  • Form

    Liquid

  • Additional notes

    For maximum product recovery after thawing, centrifuge the vial before opening the cap.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 1.12% Potassium chloride, 0.95% HEPES

General Info

  • Alternative names

    • Heat shock 86 kDa
    • Heat shock protein 90kDa alpha cytosolic class A member 1
    • Heat shock protein 90kDa alpha cytosolic class B member 1
    • Heat shock protein HSP 90 alpha
    • Heat shock protein HSP 90 beta
    • Heat shock protein HSP 90-alpha
    • HS90A_HUMAN
    • HSP 84
    • HSP 86
    • Hsp 90
    • HSP86
    • HSP90A
    • HSP90AA1
    • HSP90AB1
    • HSP90B
    • HSPC1
    • HSPC2
    • HSPCAL1
    • HSPCAL4
    • Renal carcinoma antigen NY-REN-38
    see all

  • Function

    Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.

  • Sequence similarities

    Belongs to the heat shock protein 90 family.

  • Domain

    The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1.

  • Post-translational
    modifications

    ISGylated.
    S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1.

  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab157271? Please let us know so that we can cite the reference in this datasheet.

ab157271 has not yet been referenced specifically in any publications.

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