Recombinant S. cerevisiae Hsp90 protein (ab157271)

Submit an Abreview Submit a Question

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
      P02829
    • Species
      Saccharomyces cerevisiae
    • Sequence
      MASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSL SDPKQLETEPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSG TKAFMEALSAGADVSMIGQFGVGFYSLFLVADRVQVISKSNDDEQYIWES NAGGSFTVTLDEVNERIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEF VAYPIQLVVTKEVEKEVPIPEEEKKDEEKKDEEKKDEDDKKPKLEEVDEE EEKKPKTKKVKEEVQEIEELNKTKPLWTRNPSDITQEEYNAFYKSISNDW EDPLYVKHFSVEGQLEFRAILFIPKRAPFDLFESKKKKNNIKLYVRRVFI TDEAEDLIPEWLSFVKGVVDSEDLPLNLSREMLQQNKIMKVIRKNIVKKL IEAFNEIAEDSEQFEKFYSAFSKNIKLGVHEDTQNRAALAKLLRYNSTKS VDELTSLTDYVTRMPEHQKNIYYITGESLKAVEKSPFLDALKAKNFEVLF LTDPIDEYAFTQLKEFEGKTLVDITKDFELEETDEEKAEREKEIKEYEPL TKALKEILGDQVEKVVVSYKLLDAPAAIRTGQFGWSANMERIMKAQALRD SSMSSYMSSKKTFEISPKSPIIKELKKRVDEGGAQDKTVKDLTKLLYETA LLTSGFSLDEPTSFASRINRLISLGLNIDEDEETETAPEASTAAPVEEVP ADTEMEEVD
    • Molecular weight
      82 kDa
    • Amino acids
      1 to 709
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab157271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

  • Purity
    >= 99 % .

  • Form
    Liquid
  • Additional notes
    For maximum product recovery after thawing, centrifuge the vial before opening the cap.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 1.12% Potassium chloride, 0.95% HEPES

General Info

  • Alternative names
    • Heat shock 86 kDa
    • Heat shock protein 90kDa alpha cytosolic class A member 1
    • Heat shock protein 90kDa alpha cytosolic class B member 1
    • Heat shock protein HSP 90 alpha
    • Heat shock protein HSP 90 beta
    • Heat shock protein HSP 90-alpha
    • HS90A_HUMAN
    • HSP 84
    • HSP 86
    • Hsp 90
    • HSP86
    • HSP90A
    • HSP90AA1
    • HSP90AB1
    • HSP90B
    • HSPC1
    • HSPC2
    • HSPCAL1
    • HSPCAL4
    • Renal carcinoma antigen NY-REN-38
    see all
  • Function
    Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
  • Sequence similarities
    Belongs to the heat shock protein 90 family.
  • Domain
    The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1.
  • Post-translational
    modifications
    ISGylated.
    S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1.
  • Cellular localization
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

References

ab157271 has not yet been referenced specifically in any publications.

Publishing research using ab157271? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

There are currently no Customer reviews or Questions for ab157271.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up