Description

  • Product name

    Recombinant S. cerevisiae MultiDsk (array of 5 UBA domains) protein
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Saccharomyces cerevisiae
    • Predicted molecular weight

      60 kDa including tags
    • Tags

      GST tag C-Terminus
    • Additional sequence information

      5 tandem repeats of coding sequence for the yeast Dsk2 ubiquitin binding domain. Propagated in BL-21 (DE3) cells.

Specifications

Our Abpromise guarantee covers the use of ab179563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Immunoprecipitation

  • Form

    Liquid
  • Additional notes

    ab179563 is a ubiquitin-binding protein reagent, composed of an array of five UBA domains from the yeast ubiquitin-binding protein Dsk2, fused to GST. MultiDsk binds ubiquitylated substrates with unprecedented avidity, and can be used as both an affinity resin to study protein ubiquitylation, and to effectively protect ubiquitylated proteins from the action of DUBs and the proteasome in crude cell extracts. MultiDsk offers the opportunity to obtain pure, native, nontagged, ubiquitylated proteins from cell extracts whilst helping ensure the maintenance of the normal ubiquitylation state via a strong protective function, inhibiting de-ubiquitylation.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.

    pH: 8.10
    Constituents: 2.24% Potassium chloride, 0.68% Potassium phosphate, 10% Glycerol, 0.05% Triton-X-100, 0.46% Glutathione

    Contains 1X PIs(1X Protease Inhibitor mix)

General Info

  • Alternative names

    • DSK2
    • SHE4
    • Ubiquitin domain containing protein DSK2
    • Ubiquitin domain-containing protein DSK2
    • YM8021.02
    • YMR276W
    see all
  • Relevance

    Ubiquitylation is a highly diverse and complex post-translational modification for the regulation of protein function and stability. Studies of ubiquitylation have, however, been hampered by its rapid reversal in cell extracts, for example through the action of de-ubiquitylating enzymes (DUBs). MultiDsk is a novel ubiquitin-binding protein reagent and is composed of an array of five UBA domains from the yeast ubiquitin-binding protein Dsk2, fused to a proprietary tag. MultiDsk binds ubiquitylated substrates with unprecedented avidity, and can be used as both an affinity resin to study protein ubiquitylation, and to effectively protect ubiquitylated proteins from the action of DUBs and the proteasome in crude cell extracts.
  • Cellular localization

    Nuclear

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

Application
Other
HeLa cells were stimulated (+) or not (-) with 10 ng/ml of IL-1. 2 mg of cell extract was incubated with 12 ul of GST beads and 0.5 ug of the Multi-Dsk for 4 h. The beads were washed 3x with 0.5 M NaCl, 1x 10 mM Tris and then eluted into 30 ul of SDS sample buffer. The samples were run on a 4-12% gel and blotted with the indicated antibodies.

Abcam user community

Verified customer

Submitted May 07 2014

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