Key features and details
- Expression system: Escherichia coli
- Tags: GST tag C-Terminus
- Suitable for: IP
Product nameRecombinant S. cerevisiae MultiDsk (array of 5 UBA domains) protein
Expression systemEscherichia coli
Protein lengthProtein fragment
Predicted molecular weight60 kDa including tags
TagsGST tag C-Terminus
Additional sequence information5 tandem repeats of coding sequence for the yeast Dsk2 ubiquitin binding domain. Propagated in BL-21 (DE3) cells.
Our Abpromise guarantee covers the use of ab179563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab179563 is a ubiquitin-binding protein reagent, composed of an array of five UBA domains from the yeast ubiquitin-binding protein Dsk2, fused to GST. MultiDsk binds ubiquitylated substrates with unprecedented avidity, and can be used as both an affinity resin to study protein ubiquitylation, and to effectively protect ubiquitylated proteins from the action of DUBs and the proteasome in crude cell extracts. MultiDsk offers the opportunity to obtain pure, native, nontagged, ubiquitylated proteins from cell extracts whilst helping ensure the maintenance of the normal ubiquitylation state via a strong protective function, inhibiting de-ubiquitylation.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
Constituents: 2.24% Potassium chloride, 0.68% Potassium phosphate, 10% Glycerol, 0.05% Triton-X-100, 0.46% Glutathione
Contains 1X PIs(1X Protease Inhibitor mix)
- Ubiquitin domain containing protein DSK2
RelevanceUbiquitylation is a highly diverse and complex post-translational modification for the regulation of protein function and stability. Studies of ubiquitylation have, however, been hampered by its rapid reversal in cell extracts, for example through the action of de-ubiquitylating enzymes (DUBs). MultiDsk is a novel ubiquitin-binding protein reagent and is composed of an array of five UBA domains from the yeast ubiquitin-binding protein Dsk2, fused to a proprietary tag. MultiDsk binds ubiquitylated substrates with unprecedented avidity, and can be used as both an affinity resin to study protein ubiquitylation, and to effectively protect ubiquitylated proteins from the action of DUBs and the proteasome in crude cell extracts.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab179563 has been referenced in 2 publications.