Description

  • Product name

    Recombinant S. japonicum GST protein
    See all GST proteins and peptides
  • Expression system

    Escherichia coli
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Schistosoma japonicum
    • Amino acids

      1 to 226

Specifications

Our Abpromise guarantee covers the use of ab70456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol, 0.87% Sodium chloride

General Info

  • Alternative names

    • Glutathione S Transferase
    • Glutathione S-transferase class-mu 26 kDa isozyme
    • GST
    • Sj26 antigen
    • SjGST
    see all
  • Relevance

    GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

Images

  • SDS-PAGE showing GST at approximately 26kDa.

References

This product has been referenced in:

  • Hosokawa H  et al. Gata3/Ruvbl2 complex regulates T helper 2 cell proliferation via repression of Cdkn2c expression. Proc Natl Acad Sci U S A 110:18626-31 (2013). Read more (PubMed: 24167278) »
  • Hosokawa H  et al. Functionally distinct Gata3/Chd4 complexes coordinately establish T helper 2 (Th2) cell identity. Proc Natl Acad Sci U S A 110:4691-6 (2013). Read more (PubMed: 23471993) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abreviews
Application
Western blot
Western blot of this GST protein (26kDa) was successful using Rb-antiGST monoclonal antibody (GST (91G1) Rabbit mAb #2625).

Abcam user community

Verified customer

Submitted Jul 09 2014

Answer

Thank you for contacting Abcam.

The GST protein, ab70456 should work fine in a western blot, although since we have not technically tested it in this application, then we cannot guarantee that it will work. Therefore you could take advantage of our 100% Abreview testing discount program:

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



I would recommend the following primary antibody for use in western blot:

https://www.abcam.com/GST-antibody-ab9085.html



If there is anything else I can help you with, or if you would like to take advantage of out testing discount offer, then please just let me know.

Read More

Answer

I am very glad to hear that our anti-Beta Actin loading control has worked so well for you.

In regards to a loading control for your current needs, Beta Actin would only be present in serum if shearing of cells had occurred during collection so this would not make a viable control. I have come across data stating that tranferrin and alpha anti-Trypsin are usually expressed at levels ˜2.5mg/ml and ˜1.3mg/ml respectively. However, as these may vary in samples, it may be best to add a purified peptide such a GST to your sample and stain against that.

We do offer antibodies against Transferrin, alpha anti-Trypsin and GST as well as purified GST peptides.

GST (ab70456):https://www.abcam.com/GST-protein-ab70456.html

anti-GST antibody (ab92):https://www.abcam.com/GST-antibody-3G10-1B3-ab92.html

anti-alpha-anti-Trypsin antibody (ab7633):https://www.abcam.com/alpha-1-Antitrypsin-antibody-ab7633.html

anti-Tranferrin antibody (ab1223):https://www.abcam.com/Transferrin-antibody-ab1223.html



I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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