Description

  • Product name

    Recombinant SARS CoV Envelope protein
  • Purity

    > 95 % SDS-PAGE.
    Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Sequence

      MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA

Specifications

Our Abpromise guarantee covers the use of ab63311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 4.00
    Constituent: 0.082% Sodium acetate

  • Reconstitution
    Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited.

General Info

  • Alternative names

    • E
    • E protein
    • Envelope small membrane protein
    • sM protein
  • Relevance

    Component of the viral envelope that plays a central role in virus morphogenesis and assembly. May be sufficient to form virus-like particles
  • Cellular localization

    Virion membrane; Single-pass type III membrane protein (Potential). Golgi apparatus membrane; Single-pass type III membrane protein (Potential). Note: Largely membrane-embedded. The N-terminus could be located within the membrane near the cytoplasmic side since no part of the protein is aparently exposed on the virion outside. The large hydrophobic domain could form a hairpin, looping back through the membrane. There also may be a second hydrophobic domain integrated into membranes.

Images

  • 1. MW marker – 14, 21, 31, 45, 66, 97 kDa
    2. reduced and heated sample, 5μg/lane
    3. non-reduced and non-heated sample, 5μg/lane

References

ab63311 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab63311.
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