The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 4.00 Constituent: 0.082% Sodium acetate
Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited.
Envelope small membrane protein
Component of the viral envelope that plays a central role in virus morphogenesis and assembly. May be sufficient to form virus-like particles
Virion membrane; Single-pass type III membrane protein (Potential).
Golgi apparatus membrane; Single-pass type III membrane protein (Potential).
Note: Largely membrane-embedded. The N-terminus could be located within the membrane near the cytoplasmic side since no part of the protein is aparently exposed on the virion outside. The large hydrophobic domain could form a hairpin, looping back through the membrane. There also may be a second hydrophobic domain integrated into membranes.
SDS-PAGE - Recombinant SARS CoV Envelope protein (ab63311)