Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag C-Terminus
- Suitable for: ELISA, WB
Product nameRecombinant SARS spike glycoprotein (Coronavirus)
See all SARS spike glycoprotein proteins and peptides
Purity> 90 % SDS-PAGE.
Purified by affinity chromatography.
Expression systemEscherichia coli
Protein lengthProtein fragment
SequenceRVDFCGKGYH LMSFPQAAPH GVVFLHVTYV PSQERNFTTA PAICHEGKAY FPREGVFVFN GTSWFITQRN FFSPQIITTD NTFVSGNCDV VIGIINNTVY DPLQPELDSF KEELDKYFKN HTSPDVDLGD ISGINASVVN IQKEIDRLNE VAKNLNESLI DLQELGKYEQ YIKWPWYVWL GFIAGLIAIV MVTILLCCMT SCCSCLKGAC SCGSCCKFDE DDSEPVLKGV KLHYT
Predicted molecular weight27 kDa
TagsHis tag C-Terminus
Additional sequence informationE. coli recombinant containing Spike protein immunodominant regions Fusions Six histidines. This protein is from the Spike protein S1 region.
Our Abpromise guarantee covers the use of ab49046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 9% Urea, 0.32% Tris HCl, 50% Glycerol (glycerin, glycerine)
- E2 glycoprotein
- Human coronavirus spike glycoprotein
RelevanceA novel coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive stranded RNA approximately 27 to 31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab49046 has been referenced in 1 publication.
- Gasparello J et al. Sulforaphane inhibits the expression of interleukin-6 and interleukin-8 induced in bronchial epithelial IB3-1 cells by exposure to the SARS-CoV-2 Spike protein. Phytomedicine 87:153583 (2021). PubMed: 34033999