Key features and details
- Expression system: Escherichia coli
- Purity: > 80% Affinity purified
- Endotoxin level: = 5.165 Eu/mg
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE, ELISA
Product nameRecombinant Staphylococcus alpha Hemolysin protein (Active)
ab233724 is active in functional lysis assays with rabbit red blood cells.
EC50 ~0.015 µg/ml.
Purity> 80 % Affinity purified.
Purification by column chromatography.
Endotoxin level= 5.165 Eu/mg
Expression systemEscherichia coli
Protein lengthFull length protein
Predicted molecular weight33 kDa
Additional sequence informationRecombinant protein TAG-free
Our Abpromise guarantee covers the use of ab233724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C.
Constituents: PBS, 20% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Alpha hemolysin
- Alpha HL
- Alpha toxin
RelevanceStaphylococcus aureus alpha Hemolysin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. The toxin self-assembles to form first, a nonlytic oligomeric intermediate, and then, a mushroom-shaped homoheptamer structure. Heptamer oligomerization and pore formation is required for lytic activity.
Lysis of rabbit red blood cells by S. aureus Hla.
Red blood cell lysis was determined by absorbance at OD 416 nm after 30 min incubation at 37°C with Hla.
Lane 1: 5 ug.
Lane 2: 1 ug.
All lanes : Mouse anti-Alpha Toxin (6C12) monoclonal antibody at 1 µg/ml
Lane 1 :
Recombinant Staphylococcus alpha Hemolysin protein (Active) (ab233724) at 0.005 µg
Lane 2 :
Recombinant Staphylococcus alpha Hemolysin protein (Active) (ab233724) at 0.01 µg
Lane 3 :
Recombinant Staphylococcus alpha Hemolysin protein (Active) (ab233724) at 0.05 µg
All lanes : Anti-mouse IgG-HRP conjugate
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233724 has been referenced in 1 publication.
- Chu AJ et al. Nusbiarylins Inhibit Transcription and Target Virulence Factors in Bacterial Pathogen Staphylococcus aureus. Int J Mol Sci 21:N/A (2020). PubMed: 32796751