Description

  • Product name

    Recombinant Staphylococcus alpha Hemolysin protein (Active)
  • Biological activity

    ab233724 is active in functional lysis assays with rabbit red blood cells.

    EC50 ~0.015 µg/ml.

  • Endotoxin level

    = 5.165 Eu/mg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Predicted molecular weight

      33 kDa

Specifications

Our Abpromise guarantee covers the use of ab233724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

    ELISA

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C.

    Constituent: PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Alpha hemolysin
    • Alpha HL
    • Alpha toxin
    • Hla
    • Hly
    • Staphylococcus alpha HL
    • Staphylococcus alpha toxin
    see all
  • Relevance

    Staphylococcus aureus alpha Hemolysin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. The toxin self-assembles to form first, a nonlytic oligomeric intermediate, and then, a mushroom-shaped homoheptamer structure. Heptamer oligomerization and pore formation is required for lytic activity.
  • Cellular localization

    Secreted

Images

  • Lysis of rabbit red blood cells by S. aureus Hla.

    Red blood cell lysis was determined by absorbance at OD 416 nm after 30 min incubation at 37°C with Hla.

    EC50 values were found to be 0.0262 µg/mL for lot 1805010 (blue circles) and 0.0322 µg/mL for previous lot 1805007 (green circles).

  • Lane 1: 5 ug.

    Lane 2: 1 ug.

  • All lanes : Mouse anti-Alpha Toxin (6C12) monoclonal antibody at 1 µg/ml

    Lane 1 : Recombinant Staphylococcus alpha Hemolysin protein (Active) (ab233724) at 0.005 µg
    Lane 2 : Recombinant Staphylococcus alpha Hemolysin protein (Active) (ab233724) at 0.01 µg
    Lane 3 : Recombinant Staphylococcus alpha Hemolysin protein (Active) (ab233724) at 0.05 µg

    Secondary
    All lanes : Anti-mouse IgG-HRP conjugate

References

ab233724 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab233724.
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