Recombinant Staphylococcus aureus CHIPS protein (His tag) (ab226270)
Key features and details
- Expression system: Yeast
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
Description
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Product name
Recombinant Staphylococcus aureus CHIPS protein (His tag) -
Purity
> 90 % SDS-PAGE. -
Expression system
Yeast -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Staphylococcus aureus -
Sequence
FTFEPFPTNEEIESNKKMLEKEKAYKESFKNSGLPTTLGKLDERLRNYLK KGTKNSAQFEKMVILTENKGYYTVYLNTPLAEDRKNVELLGKMYKTYFFK KGESKSSYVINGPGKTNEYAY -
Predicted molecular weight
16 kDa including tags -
Amino acids
29 to 149 -
Tags
His tag N-Terminus -
Additional sequence information
Staphylococcus aureus (strain MRSA252) full length mature chain without signal peptide.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab226270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
LC-MS/MS -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- Chemotaxis inhibiting protein
- Chemotaxis inhibitory protein
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Relevance
The bacterial pathogen Staphylococcus aureus is insensitive to antimicriobial host defense peptides like defensins, protegrins, platelet microbicidial proteins and bacteriocins. Staphylococci have developed various resistance mechanisms including those specific for bacteriocins and several host defence peptides. A protein belonging to the resistance mechanism of Staphylococcus aureus is known as CHIPS ( Chemotaxis Inhibiting Protein for Staphylococcus aureus). CHIPS is a proteins that inhibits chemotaxis of neutrophils by blocking the Formyl Peptide Receptor (FPR) and C5a Receptor on neutrophils. -
Cellular localization
Secreted
Images
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Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of ab226270 could indicate that this peptide derived from Yeast-expressed Staphylococcus aureus (strain MRSA252) chp.
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Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of ab226270 could indicate that this peptide derived from Yeast-expressed Staphylococcus aureus (strain MRSA252) chp.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226270 has not yet been referenced specifically in any publications.