Key features and details
- Expression system: Escherichia coli
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Product nameRecombinant staphylococcus aureus hlgB protein (Active)
See all hlgB proteins and peptides
Cytotoxicity can be detected in human neutrophils when used in combination with hIg A or hlg C.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTLYKTTAT ADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFS KLYWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISI SNGLSGGLNGNTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNN GWGPYGRDSFHPTYGNELFLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPE FLSVLSHRQDGAKKSKITVTYQREMDLYQIRWNGFYWAGANYKNFKTRTF KSTYEIDWENHKVKLLDTKETENNK
Predicted molecular weight35 kDa
Amino acids1 to 325
Our Abpromise guarantee covers the use of ab233597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Can be stored at -20°C for 2-3 weeks.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Gamma hemolysin component B
- H gamma 1
- H gamma I
RelevanceToxin that seems to act by forming pores in the membrane of the cell. Has a hemolytic and a leucotoxic activity.
HL-60 (Human promyelocytic leukemia cell line) was differentiated into neutrophils by treatments with DMSO. Neutrophils were incubated with serial dilutions of rhIg A and rhIg B at equimolar concentration for 3 hours at 37°C with 5% CO2 and 95% humidity. Cellular viability was determined by adding XTT and incubation for additional 16 hours. Cells were centrifuged and the OD determined in the supernatants at 470/690 nm. Cells only represented by green circles.
EC50 value was found to be 1.10 nM for ab233597 in combination with hIgA.
All lanes : Anti-hIgGB polyclonal antibody at 0.5 µg/ml
Lane 1 :
Recombinant staphylococcus aureus hlgB protein (Active) (ab233597) at 0.2 µg/ml
Lane 2 :
Recombinant staphylococcus aureus hlgB protein (Active) (ab233597) at 0.1 µg/ml
Lane 3 :
Recombinant staphylococcus aureus hlgB protein (Active) (ab233597) at 0.05 µg/ml
Lane 4 :
Recombinant staphylococcus aureus hlgB protein (Active) (ab233597) at 0.025 µg/ml
All lanes : Anti-rabbit IgG-HRP
SDS-PAGE - Recombinant staphylococcus aureus hlgB protein (Active) (ab233597)
Lane 1: ab233597 at 1 µg.
Lane 2: ab233597 at 5 µg.
Denaturing and reducing conditions.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233597 has not yet been referenced specifically in any publications.