Key features and details
- Expression system: Escherichia coli
- Active: Yes
- Suitable for: Functional Studies, WB, SDS-PAGE
Product nameRecombinant staphylococcus aureus hlgC protein (Active)
Cytotoxicity can be detected in human neutrophils when used in combination with hIg B in a concentration range of 0.03-200 nM.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceANDTEDIGKGSDIEIIKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALIL KMQGFISSRTTYYNYKKTNHVKAMRWPFQYNIGLKTNDKYVSLINYLPKN KIESTNVSQTLGYNIGGNFQSAPSLGGNGSFNYSKSISYTQQNYVSEVEQ QNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKDPRDYFVPDSEL PPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTHYGN SYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN
Predicted molecular weight33 kDa
Amino acids30 to 315
Additional sequence informationThis product is the mature full length protein from aa 32 to 315. The signal peptide is not included.
Our Abpromise guarantee covers the use of ab233606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This protein is derived from a pathogenic organism, and may be involved in a disease process, consequently exposure may have adverse health effects.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C.
Constituents: PBS, 5% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
HL-60 (Human promyelocytic leukemia cell line) was differentiated into neutrophils by treatment with DMSO. Neutrophils were incubated with serial dilutions of hlg B tag-free and ab233606 at equimolar concentration for 3 hours at 37°C with 5% CO2 and 95% humidity. Cellular viability was determined by adding XTT and incubation for additional 16 hours. OD’s were determined in the supernatants at 470/690 nm. Red squares represent (ab233606) and blue squares represent hIgC his-tag. EC50 was found to be 2.84 nM for ab233606 and 5.53 nM for hIgC his-tag.
All lanes : Polyclonal hIgC antibody at 0.5 µg/ml
Lane 1 :
Recombinant staphylococcus aureus hlgC protein (Active) (ab233606) at 0.1 µg
Lane 2 :
Recombinant staphylococcus aureus hlgC protein (Active) (ab233606) at 0.05 µg
Lane 3 :
Recombinant staphylococcus aureus hlgC protein (Active) (ab233606) at 0.025 µg
All lanes : Anti-rabbit IgG-horseradish peroxidase (HRP) conjugate
SDS-PAGE analysis of ab233606 under denaturing and reducing conditions.
Lane 1: 1 µg
Lane 2: 5 µg
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233606 has not yet been referenced specifically in any publications.