Key features and details
- Expression system: Escherichia coli
- Purity: > 99% SDS-PAGE
- Endotoxin level: < 0.100 Eu/mg
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Product nameRecombinant Staphylococcus aureus Protein A
See all Protein A proteins and peptides
Purity> 99 % SDS-PAGE.
Purity: >99% by SDS-PAGE and HPLC analyses.
Endotoxin level< 0.100 Eu/mg
Expression systemEscherichia coli
Protein lengthProtein fragment
Predicted molecular weight36 kDa
Amino acids32 to 327
TagsHis tag N-Terminus
Additional sequence informationProtein A Ig binding domains with 6x His-tag on N-terminus. There is a linker between the His tag and recombinant fragment (~11 aa). The sequence cannot be shared.
Our Abpromise guarantee covers the use of ab52953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from this protein to ensure the maximum specific IgG binding. . The 6x His-tag can be used for affinity purification or for protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies.
Protein A binds to most human and mouse IgG subclasses (e.g: human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3).
It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit.
Protein A has little affinity to chicken, goat, rat and sheep.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
- gG-binding protein A
- Immunoglobulin G binding protein A
- Staphylococcal protein A
RelevanceProtein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
Cellular localizationSecreted; cell wall.
SDS-PAGE (4-20%) of Recombinant Protein A.
Lane 1-3: 5, 2.5, and 1 µg, Protein A under Reducing conditions,
Lane4: protein Ladder;
Lane5-7: 5, 2.5, and 1 µg of Protein A under Non-Reducing conditions
SEC chromatography of Protein A. The apparent molecular weight is ~ 70 KDa calculated based on SEC standard curve.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab52953 has been referenced in 2 publications.