Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, WB, SDS-PAGE
Product nameRecombinant staphylococcus aureus PVL F protein (Active)
PVL Cytotoxicity assay: Cytotoxicity can be detected in human neutrophils when used in combination with LukS-PV in a concentration range of 1-100 nM.
Purity> 95 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceAQHITPVSEKKVDDKITLYKTTATSDSDKLKISQILTFNFIKDKSYDKDT LILKAAGNIYSGYTKPNPKDTISSQFYWGSKYNISINSDSNDSVNVVDYA PKNQNEEFQVQQTVGYSYGGDINISNGLSGGGNGSKSFSETINYKQESYR TSLDKRTNFKKIGWDVEAHKIMNNGWGPYGRDSYHSTYGNEMFLGSRQSN LNAGQNFLEYHKMPVLSRGNFNPEFIGVLSRKQNAAKKSKITVTYQREMD RYTNFWNQLHWIGNNYKDENRATHTS
Predicted molecular weight36 kDa
Amino acids25 to 325
Additional sequence informationThis product is the mature full length protein from aa 25 to 325. The signal peptide is not included.
Our Abpromise guarantee covers the use of ab230258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: PBS, 5% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Panton-Valentine Leukocidin F subunit
- PVL F subunit
SDS-PAGE analysis of 1 µg (lane 1) and 5 µg (lane 2) ab230258.
Anti-PVL F polyclonal antibody at 0.5 µg/ml +
Recombinant staphylococcus aureus PVL F protein (Active) (ab230258) at 0.1 µg
Anti-rabbit IgG-HRP conjugate
Followed by TMB substrate.
Human promyelocytic leukemia cell line HL60 was differentiated into neutrophils by treatment with DMSO. Neutrophils were incubated with serial dilutions of ab230258 and LukS-PV at equimolar concentration for 3 hours at 37°C with 5% CO2 and 95% humidity. Cellular viability was determined by adding XTT and incubating for additional 16 hours. Cells were centrifuged and the OD determined in the supernatants at 470/690 nm. EC50 values were found to be 0.907 nM.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230258 has not yet been referenced specifically in any publications.