Recombinant Streptavidin protein (ab78833)

Reviews (1)Q&A (2)References (1)
SDS-PAGE - Recombinant Streptavidin protein (ab78833)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Streptavidin protein
      See all Streptavidin proteins and peptides

    • Purity

      > 95 % SDS-PAGE.

    • Expression system

      Escherichia coli

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Sequence

        MVHHHHHHDP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ

      • Predicted molecular weight

        17 kDa including tags

      • Amino acids

        25 to 183

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab78833 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid

    • Additional notes

      Concentration is determined by BCA assay

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituent: 0.242% Tris

    General Info

    • Alternative names

      • SA V1
      • SA V2
      • strepavidin
      • Streptavidin V1
      • Streptavidin V2
      see all

    • Relevance

      Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

    • Cellular localization

      Cytoplasmic

    Images

    • SDS-PAGE - Recombinant Streptavidin protein (ab78833)
      SDS-PAGE - Recombinant Streptavidin protein (ab78833)
      15% SDS-PAGE showing ab78833 at approximately 17kDa (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab78833? Please let us know so that we can cite the reference in this datasheet.

    ab78833 has been referenced in 1 publication.

    • Qin GW et al. Surface modification of permalloy (Ni80Fe20) nanoparticles for biomedical applications Journal of Nanoparticle Research 13:45-51 (2010). ELISA . PubMed:

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Streptaividin is in the tetramic form out of the bottle

     Excellent
    Abreviews
    abreview image
    Application
    Conjugation
    Gel used in the experiment is a 4-12% bis-tris invitrogen gel ran cold at 200V. The gel contains denatured and non-denatured lanes for ab78833 and ab156734. This shows that the protein is in the tetrameric form ‘out of the bottle’.

    Dr. Sam Roberts

    Verified customer

    Submitted Sep 18 2019

    Question

    I am wondering whether the His tag for this product is accessible?

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    Answer

    If you are asking whether it’s detectable with a His tag antibody, then in principle it should be. If you are asking whether it’s the cleavable form, regrettably, it isn’t.

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    Question

    Please send confirmation that the His tag is functional even though MV in front of tag.

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    Answer

    I can confirm that the His tag is functional in this peptide for complexing with Nickel. The Met and Val in front of the His tag helps the His tag for proper configuration and has no impact on the protein’s functionality.

    Read More

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