Recombinant Streptavidin protein (ab78833)

Reviews (1)Q&A (2)References (1)

Description

  • Product name

    Recombinant Streptavidin protein
    See all Streptavidin proteins and peptides

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Sequence

      MVHHHHHHDP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ

    • Predicted molecular weight

      17 kDa including tags

    • Amino acids

      25 to 183

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab78833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

    Concentration is determined by BCA assay

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituent: 0.242% Tris

General Info

  • Alternative names

    • SA V1
    • SA V2
    • strepavidin
    • Streptavidin V1
    • Streptavidin V2
    see all

  • Relevance

    Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

  • Cellular localization

    Cytoplasmic

Images

  • SDS-PAGE - Recombinant Streptavidin protein (ab78833)
    SDS-PAGE - Recombinant Streptavidin protein (ab78833)
    15% SDS-PAGE showing ab78833 at approximately 17kDa (3µg).

References

This product has been referenced in:

  • Qin GW et al. Surface modification of permalloy (Ni80Fe20) nanoparticles for biomedical applications Journal of Nanoparticle Research 13:45-51 (2010). ELISA . Read more (PubMed: ) »
See 1 Publication for this product

Publishing research using ab78833? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Streptaividin is in the tetramic form out of the bottle

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Abreviews
abreview image
Application
Conjugation
Gel used in the experiment is a 4-12% bis-tris invitrogen gel ran cold at 200V. The gel contains denatured and non-denatured lanes for ab78833 and ab156734. This shows that the protein is in the tetrameric form ‘out of the bottle’.

Dr. Sam Roberts

Verified customer

Submitted Sep 18 2019

Question

I am wondering whether the His tag for this product is accessible?

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Answer

We weren’t sure what your client means by “accessible”. If they’re asking whether it’s detectable with a His tag antibody, then in principle it should be. If they are asking whether it’s the cleavable form, regrettably, it isn’t.


I hope this is helpful.Please let me know if you have anyadditional questions.

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Question

Please send confirmation that the His tag is functional even though MV in front of tag.

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Answer

I can confirm that the His tag is functional in this peptide for complexing with Nickel. The Met and Val in front of the His tag helps the His tag for proper configuration and has no impact on the protein’s functionality. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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