Recombinant Streptavidin protein (ab78833)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Sequence
      MVHHHHHHDP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ
    • Molecular weight
      17 kDa including tags
    • Amino acids
      25 to 183
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab78833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    >95% by SDS-PAGE .

  • Form
    Liquid
  • Additional notes

    Concentration is determined by BCA assay

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituent: 0.242% Tris

General Info

  • Alternative names
    • SA V1
    • SA V2
    • strepavidin
    • Streptavidin V1
    • Streptavidin V2
    see all
  • Relevance
    Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
  • Cellular localization
    Cytoplasmic

Images

  • SDS-PAGE - Recombinant Streptavidin protein (ab78833)
    SDS-PAGE - Recombinant Streptavidin protein (ab78833)
    15% SDS-PAGE showing ab78833 at approximately 17kDa (3µg).

References

This product has been referenced in:
  • Qin GW et al. Surface modification of permalloy (Ni80Fe20) nanoparticles for biomedical applications Journal of Nanoparticle Research 13:45-51 (2010). ELISA . Read more (PubMed: ) »
See 1 Publication for this product

Publishing research using ab78833? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

I am wondering whether the His tag for this product is accessible?

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Answer

We weren’t sure what your client means by “accessible”. If they’re asking whether it’s detectable with a His tag antibody, then in principle it should be. If they are asking whether it’s the cleavable form, regrettably, it isn’t.


I hope this is helpful.Please let me know if you have anyadditional questions.

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Question

Please send confirmation that the His tag is functional even though MV in front of tag.

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Answer

I can confirm that the His tag is functional in this peptide for complexing with Nickel. The Met and Val in front of the His tag helps the His tag for proper configuration and has no impact on the protein’s functionality. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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