Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Molecular weight

      56 kDa

Associated products

Specifications

Our Abpromise guarantee covers the use of ab123480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Binds biotin noncovalently and with high affinity (Kd ~10-15 M).

    The activity of recombinant Streptavidin is ~17 units/mg, where one unit is defined as the amount of protein required to bind 1.0 µg of D- biotin.

  • Applications

    Western blot

    Flow Cytometry

    In situ hybridization

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

    The extinction coefficient of Streptavidin is E1% 280=32.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute the powder with desired buffer. The reconstituted solution will be stable for at least six months at -20°C. The reconstituted solution may also be divided into aliquots to avoid repeated freeze-thaw cycles.

General Info

  • Alternative names

    • SA V1
    • SA V2
    • strepavidin
    • Streptavidin V1
    • Streptavidin V2
    see all
  • Relevance

    Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
  • Cellular localization

    Cytoplasmic

Images

  • A---native streptavidin, 2.5µg/lane

    B---recombinant streptavidin, 2.5µg/lane

    All samples were mixed with reducing sample buffer and run in SDS buffer.

References

ab123480 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer


ab123480 is the full length streptavidin protein and actively binds biotin.

The laboratory has not had experience using streptavidin protein, followed by primary anti-streptavidin and secondary labeling via western blot. They suggested using streptavidin conjugated to HRP instead for biotin detection, but based on your description of your experiment you wanted to detect the steptavidin with an anti-streptavidin antibody. As we don't have experience with this method of detection, we cannot guarantee it will work and it will require optimization, but we recommend first running the gel, transfer, and then incubate the membrane with streptavidin followed by the primary anti-streptavidin antibody.

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