Product nameRecombinant Streptavidin protein (Active)
See all Streptavidin proteins and peptides
Binds biotin noncovalently and with high affinity (Kd ~10-15 M).
The activity of recombinant Streptavidin is ~17 units/mg, where one unit is defined as the amount of protein required to bind 1.0 µg of D- biotin.
Expression systemEscherichia coli
Protein lengthFull length protein
Predicted molecular weight56 kDa
Our Abpromise guarantee covers the use of ab123480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
In situ hybridization
Additional notesThe extinction coefficient of Streptavidin is E1% 280=32.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute the powder with desired buffer. The reconstituted solution will be stable for at least six months at -20°C. The reconstituted solution may also be divided into aliquots to avoid repeated freeze-thaw cycles.
- SA V1
- SA V2
RelevanceStreptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab123480 has not yet been referenced specifically in any publications.