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    recombinant-streptavidin-protein-fitc-ab277701.pdf

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Recombinant Streptavidin protein (FITC) (ab277701)

  • Datasheet
  • SDS
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Flow Cytometry - Recombinant Streptavidin protein (FITC) (ab277701)
  • ELISA - Recombinant Streptavidin protein (FITC) (ab277701)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Suitable for: Flow Cyt, ELISA

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Description

  • Product name

    Recombinant Streptavidin protein (FITC)
    See all Streptavidin proteins and peptides
  • Purity

    > 90 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

    P22629
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    Specifications

    Our Abpromise guarantee covers the use of ab277701 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Flow Cytometry

      ELISA

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C. Store In the Dark.

      pH: 7.4
      Preservative: 0.1% Sodium azide
      Constituent: 99.9% PBS

    General Info

    • Alternative names

      • SA V1
      • SA V2
      • strepavidin
      • Streptavidin V1
      • Streptavidin V2
      see all
    • Relevance

      Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
    • Cellular localization

      Cytoplasmic

    Images

    • Flow Cytometry - Recombinant Streptavidin protein (FITC) (ab277701)
      Flow Cytometry - Recombinant Streptavidin protein (FITC) (ab277701)

      BALB/c mouse splenocytes were stained with a Rat Anti-Mouse CD45R-Biotinylated antibody, followed by ab277701.

    • ELISA - Recombinant Streptavidin protein (FITC) (ab277701)
      ELISA - Recombinant Streptavidin protein (FITC) (ab277701)

      ELISA plate was coated with Goat Anti-Human IgG-Biotin and purified/unlabeled Rat IgG1κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted ab277701.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab277701? Please let us know so that we can cite the reference in this datasheet.

    ab277701 has not yet been referenced specifically in any publications.

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