Recombinant Streptavidin protein (FITC) (ab277701)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: Flow Cyt, ELISA
Description
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Product name
Recombinant Streptavidin protein (FITC)
See all Streptavidin proteins and peptides -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Specifications
Our Abpromise guarantee covers the use of ab277701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Flow Cytometry
ELISA
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C. Store In the Dark.
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: 99.9% PBS
General Info
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Alternative names
- SA V1
- SA V2
- strepavidin
see all -
Relevance
Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. -
Cellular localization
Cytoplasmic
Images
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Flow Cytometry - Recombinant Streptavidin protein (FITC) (ab277701)
BALB/c mouse splenocytes were stained with a Rat Anti-Mouse CD45R-Biotinylated antibody, followed by ab277701.
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ELISA - Recombinant Streptavidin protein (FITC) (ab277701)ELISA plate was coated with Goat Anti-Human IgG-Biotin and purified/unlabeled Rat IgG1κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted ab277701.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab277701 has not yet been referenced specifically in any publications.