Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: Flow Cyt, ELISA
Product nameRecombinant Streptavidin protein (FITC)
See all Streptavidin proteins and peptides
Purity> 90 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthFull length protein
ConjugationFITC. Ex: 493nm, Em: 528nm
Our Abpromise guarantee covers the use of ab277701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C. Store In the Dark.
Preservative: 0.1% Sodium azide
Constituent: 99.9% PBS
- SA V1
- SA V2
RelevanceStreptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
BALB/c mouse splenocytes were stained with a Rat Anti-Mouse CD45R-Biotinylated antibody, followed by ab277701.
ELISA plate was coated with Goat Anti-Human IgG-Biotin and purified/unlabeled Rat IgG1κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted ab277701.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab277701 has not yet been referenced specifically in any publications.