Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, SDS-PAGE, Functional Studies
Product nameRecombinant Streptokinase protein
Fully biologically active when compared to standard.
The specific biological activity measured by the ability of fibrin lysis in agarose plate was found to be 80000U/mg.
Purity> 95 % SDS-PAGE.
>95% by HPLC analysis.
Endotoxin level< 1.000 Eu/µg
Expression systemEscherichia coli
Protein lengthFull length protein
Predicted molecular weight47 kDa
Amino acids27 to 440
Additional sequence information(Streptococcus). Single non-glycosylated polypeptide chain containing 414 amino acids. This product is the mature full length protein from aa 27 to 440. The signal peptide is not included.
Our Abpromise guarantee covers the use of ab201364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C long term. Avoid freeze / thaw cycle.
Constituent: 100% PBS
Lyophilized from a 0.2µm filtered solution.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionBriefly centrifuge the vial prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
RelevanceStreptokinase is a metallo-enzyme produced by various strains of Streptococcus that can liquify blood clots by converting plasminogen to plasmin. Streptokinase is not a protease but it activates plasminogen by complexing with it. It is thought to prevent the formation of effective fibrin barriers around the site of infection, thereby contributing to the invasiveness of the cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab201364 has not yet been referenced specifically in any publications.