Recombinant Superoxide Dismutase 1 protein (ab112193)



  • Nature
  • Source
    Escherichia coli


Our Abpromise guarantee covers the use of ab112193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Purity
    > 95 % SDS-PAGE.

  • Form
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Reconstitution
    Reconstitute in H2O to a concentration >100 µg/ml. The solution can then be diluted into other aqueous buffers.

General Info

  • Alternative names
    • ALS
    • ALS1
    • Amyotrophic lateral sclerosis 1 adult
    • Cu/Zn SOD
    • Cu/Zn superoxide dismutase
    • Epididymis secretory protein Li 44
    • HEL S 44
    • Homodimer
    • hSod1
    • Indophenoloxidase A
    • IPOA
    • Mn superoxide dismutase
    • SOD
    • SOD soluble
    • SOD1
    • SOD2
    • SODC
    • Superoxide dismutase [Cu-Zn]
    • Superoxide dismutase 1
    • Superoxide dismutase 1 soluble
    • Superoxide dismutase Cu Zn
    • Superoxide dismutase cystolic
    see all
  • Function
    Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
  • Involvement in disease
    Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
  • Sequence similarities
    Belongs to the Cu-Zn superoxide dismutase family.
  • Post-translational
    Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
    The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
  • Cellular localization
    Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
  • Information by UniProt


ab112193 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Regarding the ab112193 Recombinant Superoxide Dismutase 1 protein standard for use with ab65354 Superoxide Dismutase Activity Assay Kit:  The concentration of Cu and Zn have not been measured as they are bound to the protein and come from fermenta...

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Thank you for your enquiry.

I appreciate your patience. It has taken some time to confirm details with the laboratories.

This kit is not originally designed to be run with a standard curve each time. However, if you wish to run a stan...

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The ab65354 Superoxide Dismutase Activity Colorimetric Assay Kit can be used without a standard since it is reporting as % inhibition in your sample. Blank 1 does not contain any inhibition, and your sample will have some percentage of inhibition. Bla...

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We recommend use of ab112193 SOD protein as standard for this kit.

Thank you for your enquiry.

I have investigated this and can confirm that we would recommend to use ab112193 SOD protein if you need to use a standard with the ab65354 kit. I can recommend to review the online datasheet for further informati...

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Thank you for contacting Abcam.

Recombinant Human Cu/Zn Superoxide Dismutase produced in E.Coli is a homodimer, non-glycosylated polypeptide chain containing 2 x 154 amino acids and having a total molecular mass of 31,608 Dalton.

The ...

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