Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA
    • Molecular weight
      6 kDa
    • Amino acids
      40 to 89

Associated products

Specifications

Our Abpromise guarantee covers the use of ab9587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 106 units/mg.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form
    Lyophilised
  • Additional notes
    The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is < 0.2 ng/ml, corresponding to a specific activity of > 5 x 106 units/mg.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with 100ul of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.

General Info

  • Alternative names
    • EGF like TGF
    • EGF-like TGF
    • ETGF
    • TFGA
    • TGF A
    • TGF type 1
    • TGF-alpha
    • Tgfa
    • TGFA_HUMAN
    • Transforming growth factor alpha
    • Transforming growth factor alpha precursor
    • Wa1
    • Waved 1
    see all
  • Function
    TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
  • Tissue specificity
    Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines.
  • Sequence similarities
    Contains 1 EGF-like domain.
  • Cellular localization
    Cell membrane and Secreted > extracellular space.
  • Information by UniProt

References

This product has been referenced in:
  • Hobor S  et al. TGFa and amphiregulin paracrine network promotes resistance to EGFR blockade in colorectal cancer cells. Clin Cancer Res 20:6429-38 (2014). Read more (PubMed: 24916700) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you once again for your enquiry. I am pleased to forward you some futher procedure details for testing of ab in cell culture. I hope this will be helpful to you. 1. Resuspend 3T3 cells (passage#38) in regular culture media with 10% calf serum. Transfer 100ul culture/well to assay plate (5,000 cells/well). Incubate overnight. 2. Complete media change. Replace with 100ul media containing 0.5% calf serum. Incubate overnight. 3. Complete media change. Replace with media containing 0.5% calf serum. 4. Serial dilute h-TGF-alpha in media with 0.5% calf serum. 5. Transfer 100ul diluted cytokine cells to assay plate. Volume of each well is 200ul, containing 0.5% calf serum and h-TGF-alpha. Incubate 45 hours. 6. Add 20ul of Substrate Cell Titer 96 Aqueous One Solution Reagent to each well. 7. Incubate 37oC and read at OD 490nm. Should you have any further questions, please do not hesitate to contact us.

Read More

Answer

Thank you for your enquiry and thank you very much for your patience. I do apologize for the delay, it some time to get this information from the originator of these peptides. For each of the products listed, included is the concentration of each product prior to vialing and the volume required for each sample size listed. Ab9863: 10mg/ml 50ul/vial = 500ug Ab9587: 1mg/ml 100ul/vial = 100ug Ab9596: 2mg/ml 25ul/vial = 50ug If you have any additional questions, please contact us again.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up