Recombinant Tobacco Osmotin protein (His tag) (ab224842)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE
Description
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Product name
Recombinant Tobacco Osmotin protein (His tag) -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Tobacco -
Sequence
ATIEVRNNCPYTVWAASTPIGGGRRLDRGQTWVINAPRGTKMARVWGRTN CNFNAAGRGTCQTGDCGGVLQCTGWGKPPNTLAEYALDQFSGLDFWDISL VDGFNIPMTFAPTNPSGGKCHAIHCTANINGECPRELRVPGGCNNPCTTF GGQQYCCTQGPCGPTFFSKFFKQRCPDAYSYPQDDPTSTFTCPGGSTNYR VIFCPNGQAHPNFPLEMPGSDEVAK -
Predicted molecular weight
40 kDa including tags -
Amino acids
22 to 246 -
Tags
His tag N-Terminus -
Additional sequence information
This product is the mature full length protein from aa 22 to 246 with 6xHis-SUMO tag at the N-terminus. The signal peptide is not included.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab224842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Mass Spectrometry
SDS-PAGE
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Mass spectrometry
LC-MS/MS -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Constituents: 50% Glycerol (glycerin, glycerine), Tris buffer
General Info
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Alternative names
- AP24
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab224842 with 5% enrichment gel and 15% separation gel.
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Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab224842 could indicate that this peptide derived from E.coli-expressed tobacco Osmotin protein.
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Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab224842 could indicate that this peptide derived from E.coli-expressed tobacco Osmotin protein.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab224842 has not yet been referenced specifically in any publications.