Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant vaccinia virus B18R protein (Active)
See all B18R proteins and peptides
Immobilized Human IFN-α1a at 2μg/mL (100 µL/well), can bind ab219894 with a linear range of 0.8-6 ng/mL.
Purity> 95 % SDS-PAGE.
Endotoxin level< 1.000 Eu/µg
Expression systemHEK 293 cells
Protein lengthFull length protein
SequenceHSYAIDIENEITEFFNKMRDTLPAKDSKWLNPACMFGGTMNDIAALGEPF SAKCPPIEDSLLSHRYKDYVVKWERLEKNRRRQVSNKRVKHGDLWIANYT SKFSNRRYLCTVTTKNGDCVQGIVRSHIRKPPSCIPKTYELGTHDKYGID LYCGILYAKHYNNITWYKDNKEINIDDIKYSQTGKELIIHNPELEDSGRY DCYVHYDDVRIKNDIVVSRCKILTVIPSQDHRFKLILDPKINVTIGEPAN ITCTAVSTSLLIDDVLIEWENPSGWLIGFDFDVYSVLTSRGGITEATLYF ENVTEEYIGNTYKCRGHNYYFEKTLTTTVVLE
Predicted molecular weight40 kDa including tags
Amino acids20 to 351
TagsHis tag C-Terminus
Additional sequence informationMature protein without signal peptide from Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR).
Our Abpromise guarantee covers the use of ab219894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
Constituents: 5% Trehalose, 95% PBS
Lyophilized from 0.22 µm filtered solution.
Note: 5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab219894 has not yet been referenced specifically in any publications.