Recombinant West Nile Virus (E protein) (ab48960)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Tags: His tag C-Terminus
  • Suitable for: ELISA, WB

Description

  • Product name

    Recombinant West Nile Virus (E protein)
    See all West Nile Virus (E protein) proteins and peptides

  • Biological activity

    Immunoreactive with sera from WNV -infected individuals.

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Tags

      His tag C-Terminus

    • Additional sequence information

      Tagged with six histidines.

Specifications

Our Abpromise guarantee covers the use of ab48960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Western blot

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.2
    Constituent: 0.0536% PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Envelope protein
    • Envelope protein E

  • Relevance

    West Nile Virus (WNV) is a single-stranded RNA (positive sense) virus of the family Flaviviridae, genus Flavivirus, commonly found in Africa, West Asia and the Middle East. It causes disease that is characterized by flu-like symptoms. In a small number of cases this can develop into West Nile Encephalitis (infection of the brain and spinal cord). The viral envelope consists of envelope E and membrane M proteins. The roles of the E protein include virion assembly, recognition of cell receptors, cell endosomal membrane fusion, agglutination of red blood cells, and induction of immune responses.

  • Cellular localization

    Cell Membrane

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab48960? Please let us know so that we can cite the reference in this datasheet.

ab48960 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Question



Inquiry: What is the expected molecular weight of the protein. Thank you,

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Answer



I can confirm that ab48960 Active West Nile Virus (E protein) protein fragment has a molecular weight of approximately 18 kDa.

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Question


1. What is the amino acid sequence of this recombinant protein?
2. After over expression in e coli, is any refolding attempted?
3. Have the endotoxin levels been determined for this protein?

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Answer

1. This is full length protein, however the exact sequence is considered proprietary.
2. Renaturation is performed on the column.
3. The endotoxin level has not been determined

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Question

Thank you for that information.
The customer is very interested in this products and would like to test them - do you have samples?

Thank you for your help, kind regards

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Answer

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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Question

Inquiry: Dear sirs and madams, do you have these two West Nile antigenes? And if yes, what would be the price for 100µg? · prM-Protein recombinant · Envelope (E) Protein recombinant Thanks in advance for the information.

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Answer

Thank you for contacting us.

We have the following products that may be of interest:

ab48960
West Nile Virus (E protein) protein (His tag)
www.abcam.com/ab48960
Recombinant fusion protein; West Nile Virus Envelope protein, N-terminal part of the Envelope protein, immunodominant region. Molecular weight ˜18kDa.
Expressed in E.coli

ab49049
West Nile Virus preM protein
www.abcam.com/ab49049
N-terminal region of the West Nile Virus Pre-M antigen (immunodominant region), with six histidines included.
Expressed in E.coli

I hope this information helps. Just let me know if you have further questions.

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