Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: ELISA, WB
Product nameRecombinant West Nile Virus preM protein
Purity> 95 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthProtein fragment
Additional sequence informationTagged with six histidines.
Our Abpromise guarantee covers the use of ab49049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
Constituent: 0.0536% PBS
- membrane glycoprotein M
- membrane glycoprotein precursor prM
- pre M protein
- West Nile virus
RelevanceWest Nile Virus is part of the flavivirus family, many of which are capable of causing serious illness in humans. The ssRNA + strand virus is enveloped and spherical with a diameter of 40-50 nm, similar to all flaviviruses. The virion of this virus is a nucleocapsid covered by a lipoprotein envelope. The envelope contains two proteins: the protein M and glycoprotein E. The nucleocapsid is a complex of protein C and mRNA. In immature particles, there are 60 icosaedrally organized trimeric spikes on the surface. Each spike consists of three heterodimers of envelope protein M precursor (prM) and envelope protein E. Inside cells, viral particles will contain a preM particle and E in a complex that matures through proteolytic cleavage and processing in the Golgi apparatus. WNV has three different effects on humans, the first is asymptomatic infection; the second is a mild febrile syndrome termed West Nile Fever; the third is a neuroinvasive disease termed West Nile meningitis or encephalitis. In infected individuals the ratio between the three states is roughly 110:30:1. The main route of human infection is through the bite of an infected mosquito.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab49049 has not yet been referenced specifically in any publications.