Recombinant Western Equine Encephalitis Virus E3E2 protein (His tag) (ab233725)

Description

Specifications

Our Abpromise guarantee covers the use of ab233725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Western blot

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C.

    Constituents: PBS, 10% Glycerol

    Supplemented with arginine and glutamic acid.

Images

  • All lanes : Anti-Western Equine Encephalitus Virus E2 rabbit polyclonal at 0.25 µg/ml

    Lane 1 : Recombinant Western Equine Encephalitis Virus E3E2 protein (His tag) (ab233725) at 0.05 µg
    Lane 2 : Recombinant Western Equine Encephalitis Virus E3E2 protein (His tag) (ab233725) at 0.1 µg
    Lane 3 : Recombinant Western Equine Encephalitis Virus E3E2 protein (His tag) (ab233725) at 0.2 µg

    Secondary
    All lanes : Anti-rabbit IgG-HRP


    TMB substrate

  • SDS-PAGE analysis of ab233725 under denaturing and reducing conditions.

    Lane 1: 1 µg

    Lane 2: 5 µg

     

  • Plate was coated with ab233725 starting at 800 ng/well, serially diluted in DPBS.  Washed plate was detected using Rabbit Anti-WEEV E2 pAb at 1 µg/mL. OD650 is reported. 

References

ab233725 has not yet been referenced specifically in any publications.

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