Recombinant Wheat Gliadin protein (His tag) (ab124981)

We received the following information from the lab regarding ab124981 :

The protein sequence was not designed in respect to the question whether the fragment shows sequence homologies with alpha/beta gliadin.

We cannot exclude that an anti-alpha/beta gliadin antibody recognizes this gliadin protein, but we also cannot confirm this. This must be evaluated by the customer.

As regards to thesecond question: The His-tag is attached to the C-terminus of the gliadin fragment, so the free C-terminal end is simply 'relocated' to the first His.

I hope this information is of help to you.

No, unfortunately we do not have an alpha/beta gliadin, just this gamma gliadin fragment. I have not heard back from the laboratory that supplies the antibody ab50602 to us regarding whether reactivity with gamma gliadin has be assessed. I think it is unlikely, given the differences between the amino acid sequences of alpha/beta and gamma. Here are links to them:

http://www.uniprot.org/uniprot/P02863

http://www.uniprot.org/uniprot/P21292

In any case, I am also still waiting to find out from the producer of the gamma gliadin fragment, ab124981, if it has any NH2 groups. I see three lysine residues in the full-length gamma gliadin sequence, but I do not know if they are also in the fragment. The terminus should be available for conjugation, in any case. I will send what I find out.

I am looking into the possibility of ab50602 cross-reacting with the gamma gliadin fragment ab124981. The antibody is raised against alpha/beta gliadin, which according to my alignment, does not share much sequence with gamma gliadin. I have asked the lab that produces the antibody to confirm and I will forward what they report.

I will also look into whether the fragment has any available COOH groups available for conjugation. The C terminus is occupied by the His tag but there may be glutamate or aspartate residues available.