Question (43715) | Recombinant Wheat Gliadin protein (His tag) (ab124981)
Go to datasheet (ab124981)Do you have any alpha/beta gliadin I can purchase that would cross-react with antibody Anti-Gliadin antibody (ab50602)?
In addition, I'm asking if there are any NH2 groups on the ab124981 protein available for conjugation.
Abcam community
Verified customer
Asked on Apr 13 2012
No, unfortunately we do not have an alpha/beta gliadin, just this gamma gliadin fragment. I have not heard back from the laboratory that supplies the antibody ab50602 to us regarding whether reactivity with gamma gliadin has be assessed. I think it is unlikely, given the differences between the amino acid sequences of alpha/beta and gamma. Here are links to them:
http://www.uniprot.org/uniprot/P02863
http://www.uniprot.org/uniprot/P21292
In any case, I am also still waiting to find out from the producer of the gamma gliadin fragment, ab124981, if it has any NH2 groups. I see three lysine residues in the full-length gamma gliadin sequence, but I do not know if they are also in the fragment. The terminus should be available for conjugation, in any case. I will send what I find out.
Answered on Apr 13 2012
