Overview

  • Product name

    Anti-Recoverin antibody [6A55CD6]
    See all Recoverin primary antibodies
  • Description

    Mouse monoclonal [6A55CD6] to Recoverin
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, ICC/IF, WB, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Sheep, Cow, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Recoverin.

  • Positive control

    • ab31928 gave a positive result in Mouse Retina Whole Cell Lysate.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab31928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 19361860
WB Use a concentration of 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
IHC-FoFr Use at an assay dependent concentration. See Abreview.

Target

Images

  • All lanes : Anti-Recoverin antibody [6A55CD6] (ab31928) at 5 µg/ml

    Lane 1 : Mouse Retina Whole Cell Lysate
    Lane 2 : Human Retina Normal Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG H&L Pre-Adsorbed (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa


    Exposure time: 8 minutes
  • ab31928 staining Recoverin in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue underwent fixation in paraformaldehyde without permeation and blocking steps. The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to mouse IgG, diluted 1/1000 was used as secondary.

    See Abreview

References

This product has been referenced in:

  • Liu Y  et al. Regulated differentiation of WERI-Rb-1 cells into retinal neuron-like cells. Int J Mol Med 40:1172-1184 (2017). Read more (PubMed: 28848998) »
  • Redenti S  et al. Engineering retinal progenitor cell and scrollable poly(glycerol-sebacate) composites for expansion and subretinal transplantation. Biomaterials 30:3405-14 (2009). ICC/IF ; Mouse . Read more (PubMed: 19361860) »
See all 2 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for taking the time to complete our questionnaires and contact us. I am sorry to hearthe customer has had difficulty obtaining satisfactory results from these antibodies.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

To confirm with you, four antibodies are mentioned in your email. However, 2 copies of the questionnaire for ab124025 have been provided and there is no questionnaire for ab11259.

I can provide a response for ab94585,ab124025and ab31928. If you require further help regarding ab11529, please do not hesitate to send the questionnaire and I will be pleased to help.

I would like to reassure you that ab94585 and ab31928 are tested and covered by our 6 month guarantee for use in IHC-Fr and mouse samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement. Regarding ab124025, this is tested and guaranteed in mouse, but has not been tested in frozen sections. Could you confirm if the customer has tried paraffin sections, for which we can provide the guarantee?

Reviewing this case, I would like to offer some suggestions to help optimize the results from ab94585,ab124025and ab31928. I would also appreciate if you can confirm some further details. There are similar comments to provide for all three antibodies:

1. Fixation 1 hour for frozen sections is a very long time. For Frozen sections, we recommend fixation for 10 minutes only. Otherwize there may be too much protein crosslinking from the PFA, and the epitope may not be accessible to the antibody.

2. As far as I am aware, Recoverin, GABA A Receptor alpha 1 and Neurokinin 3 Receptor NK3 are all membrane or membraneassociated protein.Detection of thesewill not require permeabilization. Indeed, Triton is a strong detergent and can disrupt membrane proteins and eptiopes, which can significantly affect the staining. Therefore, I can suggest to remove the permeabilization step.

3. I can recommend to use a more gentle detergent, such as Tween 20 in the other buffers, try 0.1% - 0.2% Tween.

4. I recommend to include 0.1 - 0.2% tween in the wash buffer, this will help to wash away any excess antibody.

5. We recommend not to mix blocking agents in an experiment. try serum only.

6. Could you confirm, are the current vials of secondary antibodies working well with other primary antibodies? What were the results of the no primary controls? The concentration of secondary antibody may need to be reduced to help optimize the results.

I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details, including information regarding ab11259.

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Answer

It was my pleassure to help you find the information that you needed. When looking at other sources please remember that at Abcam, providing quality service is just as important as providing quality products. Our products are guaranteed under our Abpromise®, which ensures that you can trust our products to perform as stated on the datasheets, or we will offer a replacement, credit, or refund.

The Abcam Abpromise® guarantee:
- 100% Scientific and Customer Support for any product you buy from Abcam or one of our authorized distributors.
- We guarantee our products work in the tested species and applications stated on the datasheet.
- We will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase.
- We investigate any quality concerns raised by customers to ensure our catalog contains products that perform to the highest standards.

Please note these conditions to our Abpromise®:
- Protocol information must be provided in order for the claim to be validated.
- Antibodies tested in recombinant samples only are not guaranteed for use on endogenous samples.
- “Predicted to react” information on the datasheets is provided for reference only and these species are not guaranteed.
- Fast Track antibodies are covered for ELISA against the immunizing peptide only.

Please let me know if you have any questions or there are other way in which Abcam might help.

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Answer

Thank you for contacting us.

This product is Protein G purified.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (cells from cornea)
Specification
cells from cornea
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Sep 16 2010

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No

Dr. Sophie Pezet

Verified customer

Submitted Jan 28 2009

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