Key features and details
- Mouse monoclonal [6A55CD6] to Recoverin
- Suitable for: WB, IHC-FoFr
- Reacts with: Mouse
- Isotype: IgG2a
Product nameAnti-Recoverin antibody [6A55CD6]
DescriptionMouse monoclonal [6A55CD6] to Recoverin
Tested applicationsSuitable for: WB, IHC-FoFrmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Sheep, Cow, Human
Recombinant full length protein corresponding to Human Recoverin.
- ab31928 gave a positive result in Mouse Retina Whole Cell Lysate.
This antibody clone is manufactured by Abcam.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
Our Abpromise guarantee covers the use of ab31928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).|
|IHC-FoFr||Use at an assay dependent concentration.|
FunctionSeems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse.
Tissue specificityRetina and pineal gland.
Sequence similaritiesBelongs to the recoverin family.
Contains 4 EF-hand domains.
- Information by UniProt
- 23 kDa photoreceptor cell-specific protein antibody
- Cancer associated retinopathy protein antibody
- Cancer-associated retinopathy protein antibody
All lanes : Anti-Recoverin antibody [6A55CD6] (ab31928) at 5 µg/ml
Lane 1 : Mouse Retina Whole Cell Lysate
Lane 2 : Human Retina Normal Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Mouse IgG H&L Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Exposure time: 8 minutes
ab31928 staining Recoverin in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue underwent fixation in paraformaldehyde without permeation and blocking steps. The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to mouse IgG, diluted 1/1000 was used as secondary.
ab31928 has been referenced in 2 publications.
- Liu Y et al. Regulated differentiation of WERI-Rb-1 cells into retinal neuron-like cells. Int J Mol Med 40:1172-1184 (2017). PubMed: 28848998
- Redenti S et al. Engineering retinal progenitor cell and scrollable poly(glycerol-sebacate) composites for expansion and subretinal transplantation. Biomaterials 30:3405-14 (2009). ICC/IF ; Mouse . PubMed: 19361860