Anti-RECQ1 antibody (ab151501)
Key features and details
- Rabbit polyclonal to RECQ1
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-RECQ1 antibody
See all RECQ1 primary antibodies -
Description
Rabbit polyclonal to RECQ1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 400-649 of Human RECQ1 (Uniprot: P46063).
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Positive control
- WB: HEK-293T and NIH 3T3 whole cell lysates; IHC-P: HumanU87 xenograft tissue; IF: HeLa cells; IP: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 72 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated retrieval with Tris-EDTA buffer pH 8
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IP |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 72 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated retrieval with Tris-EDTA buffer pH 8 |
IP
1/100 - 1/500. |
Target
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Function
DNA helicase that may play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. -
Tissue specificity
High expression in heart, lung, skeletal muscle and kidney, low expression in brain. -
Sequence similarities
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5965 Human
- Entrez Gene: 19691 Mouse
- Omim: 600537 Human
- SwissProt: P46063 Human
- SwissProt: Q9Z129 Mouse
- Unigene: 235069 Human
- Unigene: 27407 Mouse
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Alternative names
- ATP dependent DNA helicase Q1 antibody
- ATP-dependent DNA helicase Q1 antibody
- DNA helicase antibody
see all
Images
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Anti-RECQ1 antibody (ab151501) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Predicted band size: 72 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded human U87 xenograft tissue staining RECQ1 with ab151501 at 1/100 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunoprecipitation of RecQ1 protein from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract using 5 μg of ab151501.
IP Antibody sample:
1- Control IgG;
2- ab151501.
Western blot analysis was performed using ab151501, followed by anti-Rabbit IgG antibody.
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Anti-RECQ1 antibody (ab151501) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 72 kDa7.5% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab151501 has been referenced in 4 publications.
- Abu-Libdeh B et al. RECON syndrome is a genome instability disorder caused by mutations in the DNA helicase RECQL1. J Clin Invest 132:N/A (2022). PubMed: 35025765
- Zhang J et al. RECQ1 Promotes Stress Resistance and DNA Replication Progression Through PARP1 Signaling Pathway in Glioblastoma. Front Cell Dev Biol 9:714868 (2021). PubMed: 34381789
- Liu NN et al. Endogenous Bos taurus RECQL is predominantly monomeric and more active than oligomers. Cell Rep 36:109688 (2021). PubMed: 34496242
- Bakr A et al. ID3 promotes homologous recombination via non-transcriptional and transcriptional mechanisms and its loss confers sensitivity to PARP inhibition. Nucleic Acids Res 49:11666-11689 (2021). PubMed: 34718742