Anti-RECQ1 antibody (ab151501)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RECQ1 antibody
See all RECQ1 primary antibodies -
Description
Rabbit polyclonal to RECQ1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 400-649 of Human RECQ1 (Uniprot: P46063).
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Positive control
- 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji and NIH 3T3 whole cell lysates; HeLa cells; HumanU87 xenograft tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab151501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 72 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated retrieval with Tris-EDTA buffer pH 8 | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Relevance
The protein encoded by this gene is a member of the RecQ DNA helicase family. DNA helicases are enzymes involved in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Some members of this family are associated with genetic disorders with predisposition to malignancy and chromosomal instability. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 5965 Human
- Entrez Gene: 19691 Mouse
- Omim: 600537 Human
- SwissProt: P46063 Human
- SwissProt: Q9Z129 Mouse
- Unigene: 235069 Human
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Alternative names
- ATP dependent DNA helicase Q1 antibody
- DNA helicase Q1 like antibody
- DNA helicase RecQ like type 1 antibody
see all
Images
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Western blot - Anti-RECQ1 antibody (ab151501)Anti-RECQ1 antibody (ab151501) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 72 kDa
7.5% SDS PAGE -
Western blot - Anti-RECQ1 antibody (ab151501)Anti-RECQ1 antibody (ab151501) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 72 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-RECQ1 antibody (ab151501)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells, labeling RECQ1 with ab151501 at 1/100 dilution. Lower image is merged with DNA probe. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQ1 antibody (ab151501)Immunohistochemical analysis of paraffin-embedded Human U87 xenograft tissue labeling RECQ1 with ab151501 at 1/100 dilution.
Protocols
References
ab151501 has not yet been referenced specifically in any publications.
