Anti-RECQ1 antibody (ab151501)
Key features and details
- Rabbit polyclonal to RECQ1
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RECQ1 antibody
See all RECQ1 primary antibodies -
Description
Rabbit polyclonal to RECQ1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 400-649 of Human RECQ1 (Uniprot: P46063).
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Positive control
- WB: HEK-293T and NIH 3T3 whole cell lysates; IHC-P: HumanU87 xenograft tissue; IF: HeLa cells; IP: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab151501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 72 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated retrieval with Tris-EDTA buffer pH 8 | |
| IP | 1/100 - 1/500. |
Target
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Relevance
The protein encoded by this gene is a member of the RecQ DNA helicase family. DNA helicases are enzymes involved in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Some members of this family are associated with genetic disorders with predisposition to malignancy and chromosomal instability. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 5965 Human
- Entrez Gene: 19691 Mouse
- Omim: 600537 Human
- SwissProt: P46063 Human
- SwissProt: Q9Z129 Mouse
- Unigene: 235069 Human
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Alternative names
- ATP dependent DNA helicase Q1 antibody
- DNA helicase Q1 like antibody
- DNA helicase RecQ like type 1 antibody
see all
Images
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Western blot - Anti-RECQ1 antibody (ab151501)Anti-RECQ1 antibody (ab151501) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Predicted band size: 72 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQ1 antibody (ab151501)Immunohistochemical analysis of paraffin-embedded human U87 xenograft tissue staining RECQ1 with ab151501 at 1/100 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunoprecipitation - Anti-RECQ1 antibody (ab151501)Immunoprecipitation of RecQ1 protein from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract using 5 μg of ab151501.
IP Antibody sample:
1- Control IgG;
2- ab151501.
Western blot analysis was performed using ab151501, followed by anti-Rabbit IgG antibody.
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Western blot - Anti-RECQ1 antibody (ab151501)Anti-RECQ1 antibody (ab151501) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 72 kDa7.5% SDS PAGE
Protocols
References (0)
ab151501 has not yet been referenced specifically in any publications.
