Product nameAnti-RECQ1 antibody
See all RECQ1 primary antibodies
DescriptionRabbit polyclonal to RECQ1
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Species reactivityReacts with: Human
Recombinant fragment within Human RECQ1 (N terminal). The exact sequence is proprietary.
Database link: P46063
- WB: A431, HeLa and A375 whole cell lysate. IHC-P: Human breast carcinoma tissue. ICC/IF: HeLa cells. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272855 in the following tested applications.
|WB||1/500 - 1/3000.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
|IP||1/100 - 1/500.|
RelevanceThe protein encoded by this gene is a member of the RecQ DNA helicase family. DNA helicases are enzymes involved in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Some members of this family are associated with genetic disorders with predisposition to malignancy and chromosomal instability.
- ATP dependent DNA helicase Q1 antibody
- DNA helicase Q1 like antibody
- DNA helicase RecQ like type 1 antibody
All lanes : Anti-RECQ1 antibody (ab272855) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : A-375 (human malignant melanoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Paraffin-embedded human breast carcinoma tissue stained for RECQ1 using ab272855 at 1/250 dilution in immunohistochemical analysis.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15 mins.
Immunofluorescence analysis of 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling RECQ1 (green) using ab272855 at 1/500 dilution.
Counterstain: Phalloidin, a cytoskeleton marker (red).
Immunoprecipitation of RECQ1 protein from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab272855 at 5 μg. ab272855 also used for WB followed by an anti-Rabbit IgG-HRP secondary.
Lane 1: Control IgG.
Lane 2: ab272855 IP in HeLa whole cell lysate.
ab272855 has not yet been referenced specifically in any publications.