Anti-RECQ1 antibody (ab89817)
Key features and details
- Mouse polyclonal to RECQ1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-RECQ1 antibody
See all RECQ1 primary antibodies -
Description
Mouse polyclonal to RECQ1 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human RECQ1 aa 1-700. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- HeLa cells nuclear lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa.
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Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. |
Target
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Function
DNA helicase that may play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. -
Tissue specificity
High expression in heart, lung, skeletal muscle and kidney, low expression in brain. -
Sequence similarities
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5965 Human
- Omim: 600537 Human
- SwissProt: P46063 Human
- Unigene: 235069 Human
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Alternative names
- ATP dependent DNA helicase Q1 antibody
- ATP-dependent DNA helicase Q1 antibody
- DNA helicase antibody
see all
Images
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Anti-RECQ1 antibody (ab89817) at 1 µg/ml + HeLa cells nuclear extracts at 50 µg
Predicted band size: 73 kDa
Observed band size: 73 kDa -
All lanes : Anti-RECQ1 antibody (ab89817) at 1 µg/ml
Lane 1 : RECQ1 transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 73 kDa -
ab89817 at 10µg/ml staining RECQ1 in PFA-fixed, permeabilized HeLa cells by immunofluorescence.
Datasheets and documents
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Datasheet download
References (2)
ab89817 has been referenced in 2 publications.
- Derntl C et al. Application of the human estrogen receptor within a synthetic transcription factor in Trichoderma reesei. Fungal Biol Biotechnol 7:12 (2020). PubMed: 32765896
- Derntl C et al. Fusion transcription factors for strong, constitutive expression of cellulases and xylanases in Trichoderma reesei. Biotechnol Biofuels 12:231 (2019). PubMed: 31583017