Product nameAnti-RECQ1 antibody [EPR8049(2)]
See all RECQ1 primary antibodies
DescriptionRabbit monoclonal [EPR8049(2)] to RECQ1
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RECQ1 aa 600 to the C-terminus. The exact sequence is proprietary.
Database link: P46063
- HeLa, Raji, Ramos and 293T cell lysates; Human heart and thyroid gland carcinoma tissues; 293T and Ramos cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab172607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 72 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/250 - 1/500.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceThe protein encoded by this gene is a member of the RecQ DNA helicase family. DNA helicases are enzymes involved in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Some members of this family are associated with genetic disorders with predisposition to malignancy and chromosomal instability.
- ATP dependent DNA helicase Q1 antibody
- DNA helicase Q1 like antibody
- DNA helicase RecQ like type 1 antibody
All lanes : Anti-RECQ1 antibody [EPR8049(2)] (ab172607) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Raji cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 72 kDa
Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling RECQ1 with ab172607 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling RECQ1 with ab172607 at 1/50 dilution.
Immunofluorescent analysis of 293T cells labeling RECQ1 with ab172607 at 1/250 dilution.
Flow cytometric analysis of permeabilized Ramos cells labeling RECQ1 with ab172607 at 1/10 dilution (red) compared to a Rabbit IgG negative control (green).
ab172607 has not yet been referenced specifically in any publications.