Overview

  • Product name

    Anti-RECQ1 antibody [EPR8049(2)]
    See all RECQ1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8049(2)] to RECQ1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RECQ1 aa 600 to the C-terminus. The exact sequence is proprietary.
    Database link: P46063

  • Positive control

    • HeLa, Raji, Ramos and 293T cell lysates; Human heart and thyroid gland carcinoma tissues; 293T and Ramos cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab172607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance

      The protein encoded by this gene is a member of the RecQ DNA helicase family. DNA helicases are enzymes involved in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Some members of this family are associated with genetic disorders with predisposition to malignancy and chromosomal instability.
    • Cellular localization

      Nuclear
    • Database links

    • Alternative names

      • ATP dependent DNA helicase Q1 antibody
      • DNA helicase Q1 like antibody
      • DNA helicase RecQ like type 1 antibody
      • RECQ L1 antibody
      • RecQ protein like antibody
      • RecQ protein like 1 antibody
      • RECQL antibody
      • RECQL1 antibody
      see all

    Images

    • All lanes : Anti-RECQ1 antibody [EPR8049(2)] (ab172607) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : Ramos cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 72 kDa

    • Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling RECQ1 with ab172607 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling RECQ1 with ab172607 at 1/50 dilution.

    • Immunofluorescent analysis of 293T cells labeling RECQ1 with ab172607 at 1/250 dilution.

    • Flow cytometric analysis of permeabilized Ramos cells labeling RECQ1 with ab172607 at 1/10 dilution (red) compared to a Rabbit IgG negative control (green).

    References

    ab172607 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab172607.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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