Key features and details
- Rabbit polyclonal to RECQL4
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RECQL4 antibody
See all RECQL4 primary antibodies
DescriptionRabbit polyclonal to RECQL4
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
- IHC-P: Human thyroid gland tissue. ICC/IF: A431 cells. WB: RT4 and U-251 MG cells.
Previously labelled as RECQ4.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab188125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 133 kDa.|
FunctionDNA-dependent ATPase. May modulate chromosome segregation.
Tissue specificityUbiquitously expressed, with highest levels in thymus and testis.
Involvement in diseaseDefects in RECQL4 are a cause of Rothmund-Thomson syndrome (RTS) [MIM:268400]. A disease characterized by dermatological features such as atrophy, pigmentation, and telangiectasia and frequently accompanied by juvenile cataract, saddle nose, congenital bone defects, disturbances of hair growth, and hypogonadism.
Defects in RECQL4 are a cause of RAPADILINO syndrome (RAPADILINOS) [MIM:266280]. A disease characterized by radial and patellar aplasia or hypoplasia.
Defects in RECQL4 are a cause of Baller-Gerold syndrome (BGS) [MIM:218600]; also known as craniosynostosis with radial defects. BGS is an autosomal recessive syndrome characterized by short stature, craniosynostosis, absent or hypoplastic radii, short and curved ulna, fused carpal bones and absent carpals, metacarpals and phalanges. Some patients manifest poikiloderma. Cases reported as Baller-Gerold syndrome have phenotypic overlap with several other disorders, including Saethre-Chotzen syndrome. BGS is part of the clinical spectrum of Rothmund-Thomson and RAPADILINO syndromes.
Sequence similaritiesBelongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- ATP dependent DNA helicase Q4 antibody
- ATP-dependent DNA helicase Q4 antibody
- DNA helicase antibody
Paraffin embedded human thyroid gland tissue stained for RECQL4 using ab188125 at 1/50 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
Immunocytochemistry/ Immunofluorescence staining of human cell line A431 staining RECQL4 with ab188125. Antibody staining is shown in green.
All lanes : Anti-RECQL4 antibody (ab188125) at 0.4 µg/ml
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Predicted band size: 133 kDa
ab188125 has not yet been referenced specifically in any publications.