Key features and details
- Rabbit polyclonal to RECQL5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RECQL5 antibody
See all RECQL5 primary antibodies
DescriptionRabbit polyclonal to RECQL5
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- WB: A549 cell lysate. IHC-P: Human skeletal muscle tissue. ICC/IF: Human testis, cervix and cerebral cortex tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 46,49,106,109 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionMay have an important role in DNA metabolism.
Sequence similaritiesBelongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Cellular localizationCytoplasm and Nucleus > nucleoplasm.
- Information by UniProt
- ATP-dependent DNA helicase Q5 antibody
- DNA helicase antibody
- DNA helicase, RecQ like type 5 antibody
Paraffin-embedded human cerebral cortex tissue stained for RECQL5 using ab224135 at 1/50 dilution in immunohistochemical analysis.
Anti-RECQL5 antibody (ab224135) at 1/100 dilution + A549 (human lung carcinoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 46,49,106,109 kDa
Paraffin-embedded human cervix tissue stained for RECQL5 using ab224135 at 1/50 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for RECQL5 (green) using ab224135 at 4 µg/ml in ICC/IF.
Paraffin-embedded human testis tissue stained for RECQL5 using ab224135 at 1/50 dilution in immunohistochemical analysis.
ab224135 has not yet been referenced specifically in any publications.