Overview

  • Product name

  • Description

    Rabbit polyclonal to RED
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide designed between residues 507 and 557 of Human RED (NP_006074.2)

  • Positive control

    • HeLa cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    May bind to chromatin.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the RED family.
  • Developmental stage

    Expressed at similar levels in fetal and adult tissues.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • chondrosarcoma associated protein 2 antibody
    • CSA2 antibody
    • Cytokine IK antibody
    • IK antibody
    • IK cytokine down regulator of HLA II antibody
    • IK factor antibody
    • IK Gene antibody
    • prer protein antibody
    • Protein Red antibody
    • Protein RER antibody
    • RD element antibody
    • RED protein antibody
    • RED_HUMAN antibody
    • RER antibody
    see all

Images

  • All lanes : Anti-RED antibody (ab85965) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 66 kDa


    Exposure time: 10 seconds
  • 1mg HeLa whole cell lysate was immunoprecipitated using 3µg ab85965.
    20% of IP was loaded per lane and probed with with ab85965 at 1 µg/ml (lane 1) and with a control IgG (lane 2).
    Detection: chemiluminescence with an exposure time of 10 seconds.

References

ab85965 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab85965.
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