Anti-Red Blood Cells antibody (ab34858)

Submit a review Q&A (2)References (6)

Overview

  • Product name

    Anti-Red Blood Cells antibody
    See all Red Blood Cells primary antibodies

  • Description

    Rabbit polyclonal to Red Blood Cells

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Agglutinationmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue/ cell preparation: Human washed pooled Red Blood Cells.

  • General notes

    ab34858 is whole antiserum. For your information, we provide you the total protein
    concentration which is 90 mg/ml. Since the antibody is unpurified, we can only estimate its
    concentration at 1–10 mg/ml.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate
  • Concentration information loading...

  • Purification notes

    Ab34858 was prepared from polyspecific antiserum by a delipidation and defibrination.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Agglutination 1/64.

An agglutination titer against serial dilutions of washed pooled cells on a titer plate of 1:64 was observed for this product.

Target

  • Relevance

    Red blood cells are the most common type of blood cell and are the vertebrate body's principal means of delivering oxygen from the lungs or gills to body tissues via the blood.

  • Alternative names

    • Erythrocytes antibody
    • RBCs antibody

References

This product has been referenced in:

  • Quintana MDP  et al. Antibodies in children with malaria to PfEMP1, RIFIN and SURFIN expressed at the Plasmodium falciparum parasitized red blood cell surface. Sci Rep 8:3262 (2018). Read more (PubMed: 29459776) »
  • Sun J  et al. Engineered proteins with sensing and activating modules for automated reprogramming of cellular functions. Nat Commun 8:477 (2017). Read more (PubMed: 28883531) »
See all 6 Publications for this product

Publishing research using ab34858? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Product code: 34858
Inquiry: I am looking for a primary antibody to deplete erythrocytes through immunocapture (attaching the primary antibody to a magnetic particle, then capturing erythrocytes and removing them with a magnetic field). Ab34858 looks encouraging as it agglutinates, demonstrating it sees surface antigens. The 3 references refer to PfEMP1, which is a surface antigen expressed when the malarial parasite infects an RBC - can you confirm that Ab34858 binds to all human erythrocytes before I order and try it. Also if there is any information on immuno-capture, that would be very helpful

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Answer

Thank you for contacting us and your interest in our products.

Unfortunately, I do not have any further information to share in relation to the epitope recognised by the anti-Red Blood Cells antibody (ab34858). We have notourselves performed any detailed studies to elucidate the specific bound antigens.

We have not tested this whole antiserum (ab34858) for the proposed experiment or against all blood types and therefore cannot guarantee the results.This does not mean that it would not work, simply that we have not tested it ourselves.

I am sorry I could not be of more help. If you have any further questions please do not hesitate to ask.

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Question

Would like to try this antibody to stain red blood cells. Would like any additional information on the antibody.

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Answer

Thank you for your call today and for your questions about ab34858. I have checked through my internal notes and the concentration previously listed on the datasheet (90 mg/mL) is incorrect. This antibody is provided as whole antiserum and the concentration is not determined. When staining cells with a whole antiserum, we recommend a 1:50-1:100 dilution.

I've created a discount code which can be redeemed for a free primary antibody after submitting an Abreview for ab34858 in ICC (cell staining). Please see below for more details, and let me know if you have any questions or need anything else.

DISCOUNT CODE: ***
Expiration date: June 16, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab34858 in ICC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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