Anti-REDD-1/DDIT4 antibody (ab106356)
Key features and details
- Rabbit polyclonal to REDD-1/DDIT4
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-REDD-1/DDIT4 antibody
See all REDD-1/DDIT4 primary antibodies -
Description
Rabbit polyclonal to REDD-1/DDIT4 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse
Predicted to work with: Human -
Immunogen
Synthetic peptide corresponding to Human REDD-1/DDIT4 (internal sequence).
Database link: NP_061931 -
Positive control
- IHC-P: Mouse kidney tissue. ICC/IF: Mouse kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
ICC/IF for Mouse samples only |
|
IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-P for Mouse samples only |
Notes |
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ICC/IF
Use at an assay dependent concentration. ICC/IF for Mouse samples only |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. IHC-P for Mouse samples only |
Target
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Function
Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death. -
Tissue specificity
Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients (at protein level). -
Sequence similarities
Belongs to the DDIT4 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 54541 Human
- Entrez Gene: 74747 Mouse
- Omim: 607729 Human
- SwissProt: Q9NX09 Human
- SwissProt: Q9D3F7 Mouse
- Unigene: 523012 Human
- Unigene: 21697 Mouse
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Alternative names
- DDIT4 antibody
- DDIT4_HUMAN antibody
- Dig2 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab106356 has been referenced in 8 publications.
- Yang Z & Xia L Resveratrol inhibits the proliferation, invasion, and migration, and induces the apoptosis of human gastric cancer cells through the MALAT1/miR-383-5p/DDIT4 signaling pathway. J Gastrointest Oncol 13:985-996 (2022). PubMed: 35837196
- Huang P et al. Redd1 knockdown prevents doxorubicin-induced cardiac senescence. Aging (Albany NY) 13:13788-13806 (2021). PubMed: 33962393
- Jeong H et al. Caffeoyl-Prolyl-Histidine Amide Inhibits Fyn and Alleviates Atopic Dermatitis-Like Phenotypes via Suppression of NF-?B Activation. Int J Mol Sci 21:N/A (2020). PubMed: 32998341
- Huang P et al. Redd1 protects against post-infarction cardiac dysfunction by targeting apoptosis and autophagy. Int J Mol Med 44:2065-2076 (2019). PubMed: 31638187
- Mains RE et al. Changes in Corticotrope Gene Expression Upon Increased Expression of Peptidylglycine a-Amidating Monooxygenase. Endocrinology 159:2621-2639 (2018). PubMed: 29788427
- Gupta I et al. Ascorbate attenuates pulmonary emphysema by inhibiting tobacco smoke and Rtp801-triggered lung protein modification and proteolysis. Proc Natl Acad Sci U S A 113:E4208-17 (2016). PubMed: 27382160
- Zhao H et al. DNA Damage-Inducible Transcript 4 Is an Innate Surveillant of Hair Follicular Stress in Vitamin D Receptor Knockout Mice and a Regulator of Wound Re-Epithelialization. Int J Mol Sci 17:N/A (2016). PubMed: 27898044
- Polioudakis D et al. A Myc-microRNA network promotes exit from quiescence by suppressing the interferon response and cell-cycle arrest genes. Nucleic Acids Res 41:2239-54 (2013). PubMed: 23303785