Product nameAnti-REDD-1/DDIT4 antibody [EPR18716]
See all REDD-1/DDIT4 primary antibodies
DescriptionRabbit monoclonal [EPR18716] to REDD-1/DDIT4
Tested applicationsSuitable for: IP, ICC/IF, WBmore details
Species reactivityReacts with: Human
Recombinant full length protein within Human REDD-1/DDIT4 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q9NX09
- WB: HeLa and MCF7 treated with 0.5 mM CoCl2 for 6 hours whole cell lysates. ICC/IF: HeLa cells treated with 0.5mM CoCl2 for 6 hours. IP: HeLa treated with 0.5mM CoCl2 for 6 hours whole cell lysate.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab191871 in the following tested applications.
|WB||1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 25 kDa).|
FunctionInhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death.
Tissue specificityBroadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients (at protein level).
Sequence similaritiesBelongs to the DDIT4 family.
- Information by UniProt
- DDIT4 antibody
- DDIT4_HUMAN antibody
- Dig2 antibody
All lanes : Anti-REDD-1/DDIT4 antibody [EPR18716] (ab191871) at 1/1000 dilution
Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 0.5 mM CoCl2 for 6 hours whole cell lysate
Lane 3 : Untreated MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) treated with 0.5 mM CoCl2 for 6 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 25 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 100 methanol-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, untreated or treated with CoCl2 (0.5 mM) for 6 hours, labeling REDD-1/DDIT4 with ab191871 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The expression increased after treatment with CoCl2 (0.5 mM) for 6 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab191871 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
REDD-1/DDIT4 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 0.5mM CoCl2 for 6 hours whole cell lysate with ab191871 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab191871 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa treated with 0.5mM CoCl2 for 6h whole cell lysate, 10µg (Input).
Lane 2: ab191871 IP in HeLa treated with 0.5mM CoCl2 for 6h whole cell lysate.
Lane 3: Rabbit IgG,monoclonal- Isotype Control (ab172730) instead of ab191871 in HeLa treated with 0.5mM CoCl2 for 6h whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
ab191871 has not yet been referenced specifically in any publications.