redFluor™ 710 Anti-CD11b antibody [M1/70] (ab253091)
![Flow Cytometry - redFluor™ 710 Anti-CD11b antibody [M1/70] (ab253091)](https://www.abcam.com/ps/products/253/ab253091/Images/ab253091-343662-anticd11bmonoclonalantibody-m170-bonemarrowcells-flowcytometry-mouse.jpg "Flow Cytometry - redFluor™ 710 Anti-CD11b antibody [M1/70] (ab253091)")
Key features and details
- redFluor™ 710 Rat monoclonal [M1/70] to CD11b
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: redFluor™ 710
- Isotype: IgG2b
Overview
-
Product name
redFluor™ 710 Anti-CD11b antibody [M1/70]
See all CD11b primary antibodies -
Description
redFluor™ 710 Rat monoclonal [M1/70] to CD11b -
Host species
Rat -
Conjugation
redFluor™ 710 -
Specificity
The M1/70 clone is widely used as a marker for CD11b expression on mouse macrophages, granulocytes, neutrophils, and NK cells.
-
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
-
Positive control
- Flow Cyt: Mouse C57Bl/6 bone marrow cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.16% Sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from tissue culture supernatant. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
M1/70 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
- Alexa Fluor® 488 Anti-CD11b antibody [M1/70] (ab197701)
- Alexa Fluor® 647 Anti-CD11b antibody [M1/70] (ab197702)
- Alexa Fluor® 405 Anti-CD11b antibody [M1/70] (ab197703)
- PerCP/Cy5.5® Anti-CD11b antibody [M1/70] (ab210299)
- PE/Cy7® Anti-CD11b antibody [M1/70] (ab218786)
- PerCP Anti-CD11b antibody [M1/70] (ab237025)
- Anti-CD11b antibody [M1/70] - BSA and Azide free (ab237965)
- violetFluor™ 450 Anti-CD11b antibody [M1/70] (ab242266)
- FITC Anti-CD11b antibody [M1/70] (ab24874)
- PE Anti-CD11b antibody [M1/70] (ab25175)
- APC Anti-CD11b antibody [M1/70] (ab25482)
- PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
- APC/Cy7® Anti-CD11b antibody [M1/70] (ab79096)
- Anti-CD11b antibody [M1/70] (ab8878)
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab253091 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
Use at 0.25 μg per reaction. |
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. Use at 0.25 μg per reaction. |
Target
-
Function
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. -
Tissue specificity
Predominantly expressed in monocytes and granulocytes. -
Involvement in disease
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 16409 Mouse
- SwissProt: P05555 Mouse
- Unigene: 262106 Mouse
-
Alternative names
- antigen CD11b (p170) antibody
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
see all
Images
-
Flow Cytometry - redFluor™ 710 Anti-CD11b antibody [M1/70] (ab253091)
Flow cytometric analysis of mouse C57Bl/6 bone marrow cells stained for CD11b using ab253091 at 0.25 μg per reaction (blue solid line) or a rat IgG2b redFluor™ 710 isotype control (black dashed line).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab253091 has not yet been referenced specifically in any publications.