Key features and details
- redFluor™ 710 Mouse monoclonal [61D3] to CD14
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: redFluor™ 710
- Isotype: IgG1
Product nameredFluor™ 710 Anti-CD14 antibody [61D3]
See all CD14 primary antibodies
DescriptionredFluor™ 710 Mouse monoclonal [61D3] to CD14
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
The details of the immunogen for this antibody are not available.
- Flow Cyt: Human peripheral blood monocytes.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.16% Sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
Concentration information loading...
Purification notesPurified from TCS.
Light chain typekappa
Our Abpromise guarantee covers the use of ab253087 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
Use at 5 µL (0.5 µg) per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 µL. The number of cells within a samaple should be determined empirically, but typically ranges between 1x105 to 1x108 cells.
FunctionCooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Tissue specificityExpressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
Sequence similaritiesContains 11 LRR (leucine-rich) repeats.
modificationsN- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Cellular localizationCell membrane.
- Information by UniProt
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab253087 has not yet been referenced specifically in any publications.