Key features and details
- redFluor™ 710 Rat monoclonal [GK1.5] to CD4
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: redFluor™ 710
- Isotype: IgG2b
Product nameredFluor™ 710 Anti-CD4 antibody [GK1.5]
See all CD4 primary antibodies
DescriptionredFluor™ 710 Rat monoclonal [GK1.5] to CD4
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
The details of the immunogen for this antibody are not available.
- Flow Cyt: C57Bl/6 splenocytes.
If used together, the GK1.5 antibody and an alternative antibody, anti-Mouse CD4 clone RM4-5, will “compete” for binding, i.e. RM4-5 is able to block GK1.5 binding to cells. In contrast, the anti-Mouse CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells.
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
Concentration information loading...
Purification notesPurified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Light chain typekappa
Our Abpromise guarantee covers the use of ab242010 in the following tested applications.
|Flow Cyt||Use at an assay dependent concentration.
Use 0.06 µg. We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system.
FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
- Information by UniProt
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab242010 has not yet been referenced specifically in any publications.